Cell viability characterization of bioprintable blood-vessel-like cellular channels towards 3D organ fabrication

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Organ printing, or layer-by-layer additive tissue fabrication, is a revolutionary concept that has recently emerged as an interdisciplinary effort to produce three-dimensional living organs for clinical applications. However, it is a complex and challenging process to execute due to the lack of fundamental understanding of tissue and organ formation. One of the major challenges is inclusion of blood-vessel-like channels between layers to support cell viability in terms of nutrients and oxygen transport. In this research, we developed novel printable micro-fluidic channels encapsulating cells to mimic natural blood vessels. Our previous research addressed the printability of novel vessel-like micro-fluidic channels with alginate hydrogel with co-axial nozzle assembly. In this study, we further investigated the influence of bioprinting parameters on the viability of cartilage progenitor cells (CPCs) during and after printing. The results of this study revealed that quantifiable cell death could be induced by varying dispensing pressure, co-axial nozzle geometry, and biomaterial concentration. However, damaged cells were able to recover during incubation, as well as to undergo proliferation to a certain extent. These findings may serve as a guideline for optimizing our system as well as predicting cell damage in future studies.

Original languageEnglish (US)
Title of host publicationIIE Annual Conference and Expo 2013
PublisherInstitute of Industrial Engineers
Pages2299-2304
Number of pages6
StatePublished - 2013
EventIIE Annual Conference and Expo 2013 - San Juan, Puerto Rico
Duration: May 18 2013May 22 2013

Other

OtherIIE Annual Conference and Expo 2013
CountryPuerto Rico
CitySan Juan
Period5/18/135/22/13

All Science Journal Classification (ASJC) codes

  • Industrial and Manufacturing Engineering

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