Cell wall remodeling and vesicle trafficking mediate the root clock in Arabidopsis

Guy Wachsman, Jingyuan Zhang, Miguel A. Moreno-Risueno, Charles T. Anderson, Philip N. Benfey

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In Arabidopsis thaliana, lateral roots initiate in a process preceded by periodic gene expression known as the root clock. We identified the vesicle-trafficking regulator GNOM and its suppressor, ADENOSINE PHOSPHATE RIBOSYLATION FACTOR GTPase ACTIVATION PROTEIN DOMAIN3, as root clock regulators. GNOM is required for the proper distribution of pectin, a mediator of intercellular adhesion, whereas the pectin esterification state is essential for a functional root clock. In sites of lateral root primordia emergence, both esterified and de-esterified pectin variants are differentially distributed. Using a reverse-genetics approach, we show that genes controlling pectin esterification regulate the root clock and lateral root initiation. These results indicate that the balance between esterified and de-esterified pectin states is essential for proper root clock function and the subsequent initiation of lateral root primordia.

Original languageEnglish (US)
Pages (from-to)819-823
Number of pages5
JournalScience
Volume370
Issue number6518
DOIs
StatePublished - Nov 13 2020

All Science Journal Classification (ASJC) codes

  • General

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