Cellular Architecture Regulates Collective Calcium Signaling and Cell Contractility

Jian Sun, James B. Hoying, Pierre A. Deymier, Donna D. Zhang, Pak Kin Wong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A key feature of multicellular systems is the ability of cells to function collectively in response to external stimuli. However, the mechanisms of intercellular cell signaling and their functional implications in diverse vascular structures are poorly understood. Using a combination of computational modeling and plasma lithography micropatterning, we investigate the roles of structural arrangement of endothelial cells in collective calcium signaling and cell contractility. Under histamine stimulation, endothelial cells in self-assembled and microengineered networks, but not individual cells and monolayers, exhibit calcium oscillations. Micropatterning, pharmacological inhibition, and computational modeling reveal that the calcium oscillation depends on the number of neighboring cells coupled via gap junctional intercellular communication, providing a mechanistic basis of the architecture-dependent calcium signaling. Furthermore, the calcium oscillation attenuates the histamine-induced cytoskeletal reorganization and cell contraction, resulting in differential cell responses in an architecture-dependent manner. Taken together, our results suggest that endothelial cells can sense and respond to chemical stimuli according to the vascular architecture via collective calcium signaling.

Original languageEnglish (US)
Article numbere1004955
JournalPLoS computational biology
Volume12
Issue number5
DOIs
StatePublished - May 2016

Fingerprint

Contractility
Calcium Signaling
Calcium
calcium
Endothelial cells
Cell
Endothelial Cells
endothelial cells
oscillation
cells
Computational Modeling
Histamine
Oscillation
Blood Vessels
histamine
blood vessels
Cell signaling
chemical cue
cell communication
Dependent

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this

Sun, Jian ; Hoying, James B. ; Deymier, Pierre A. ; Zhang, Donna D. ; Wong, Pak Kin. / Cellular Architecture Regulates Collective Calcium Signaling and Cell Contractility. In: PLoS computational biology. 2016 ; Vol. 12, No. 5.
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Cellular Architecture Regulates Collective Calcium Signaling and Cell Contractility. / Sun, Jian; Hoying, James B.; Deymier, Pierre A.; Zhang, Donna D.; Wong, Pak Kin.

In: PLoS computational biology, Vol. 12, No. 5, e1004955, 05.2016.

Research output: Contribution to journalArticle

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AU - Hoying, James B.

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AU - Zhang, Donna D.

AU - Wong, Pak Kin

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