Cellular distribution of iron, transferrin, and ferritin in the hypotransferrinemic (Hp) mouse brain

Thomas K. Dickinson, James R. Connor

Research output: Contribution to journalArticle

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Abstract

Hypotransferrinemic (Hp) mice have a point mutation or small deletion in the transferrin (Tf) gene, resulting in defective splicing of precursor Tf mRNA. Hp animals produce 1% of normal Tf levels and require supplemental serum or purified Tf for survival. Because of the lack of endogenous brain Tf, we examined regional and cellular distributions of iron and iron regulatory proteins (Tf and ferritin) in selected brain regions of Hp mice. The regional distribution of iron, Tf, and ferritin in Hp brain was similar to normal except for the pattern of iron staining in hippocampus. The cellular distribution of iron, ferritin, and Tf was similar between Hp and normal animals. The predominant cell type, staining for Tf and iron was oligodendrocytes. Qualitative observations suggest that the number of cells staining for iron was similar between Hp and normal mice, whereas the number of Hp Tf‐positive cells was reduced. Ferritin immunostaining was similar in both cases. However, ferritin‐positive cells were preominantly astrocytes, an observation unique to mice among species studied previously. Western blot analysis revealed that Tf present in HP brain was of exogenous origin (from supplemental injections). Presumably, Tf transports the iron found in Hp oligodendrocytes. These data demonstrate that, despite reduced endogenous Hp brain Tf, iron and plasma Tf migrate or are transported to the appropriate cells (oligodendrocytes), bringing into question the role of endogenous brain Tf in extracellular iron transport.

Original languageEnglish (US)
Pages (from-to)67-80
Number of pages14
JournalJournal of Comparative Neurology
Volume355
Issue number1
DOIs
StatePublished - Apr 24 1995

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Transferrin
Ferritins
Iron
Brain
Oligodendroglia
Staining and Labeling
Iron-Regulatory Proteins
RNA Precursors
Point Mutation
Astrocytes
Hippocampus
Cell Count
Western Blotting

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "Cellular distribution of iron, transferrin, and ferritin in the hypotransferrinemic (Hp) mouse brain",
abstract = "Hypotransferrinemic (Hp) mice have a point mutation or small deletion in the transferrin (Tf) gene, resulting in defective splicing of precursor Tf mRNA. Hp animals produce 1{\%} of normal Tf levels and require supplemental serum or purified Tf for survival. Because of the lack of endogenous brain Tf, we examined regional and cellular distributions of iron and iron regulatory proteins (Tf and ferritin) in selected brain regions of Hp mice. The regional distribution of iron, Tf, and ferritin in Hp brain was similar to normal except for the pattern of iron staining in hippocampus. The cellular distribution of iron, ferritin, and Tf was similar between Hp and normal animals. The predominant cell type, staining for Tf and iron was oligodendrocytes. Qualitative observations suggest that the number of cells staining for iron was similar between Hp and normal mice, whereas the number of Hp Tf‐positive cells was reduced. Ferritin immunostaining was similar in both cases. However, ferritin‐positive cells were preominantly astrocytes, an observation unique to mice among species studied previously. Western blot analysis revealed that Tf present in HP brain was of exogenous origin (from supplemental injections). Presumably, Tf transports the iron found in Hp oligodendrocytes. These data demonstrate that, despite reduced endogenous Hp brain Tf, iron and plasma Tf migrate or are transported to the appropriate cells (oligodendrocytes), bringing into question the role of endogenous brain Tf in extracellular iron transport.",
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Cellular distribution of iron, transferrin, and ferritin in the hypotransferrinemic (Hp) mouse brain. / Dickinson, Thomas K.; Connor, James R.

In: Journal of Comparative Neurology, Vol. 355, No. 1, 24.04.1995, p. 67-80.

Research output: Contribution to journalArticle

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