Centriole Age Underlies Asynchronous Primary Cilium Growth in Mammalian Cells

Charles T. Anderson, Tim Stearns

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Primary cilia are microtubule-based sensory organelles that play important roles in development and disease [1]. They are required for Sonic hedgehog (Shh) [2-4] and platelet-derived growth factor (PDGF) [5] signaling. Primary cilia grow from the older of the two centrioles of the centrosome, referred to as the mother centriole. In cycling cells, the cilium typically grows in G1 and is lost before mitosis, but the regulation of its growth is poorly understood. Centriole duplication at G1/S results in two centrosomes, one with an older mother centriole and one with a new mother centriole, that are segregated in mitosis. Here we report that primary cilia grow asynchronously in sister cells resulting from a mitotic division and that the sister cell receiving the older mother centriole usually grows a primary cilium first. We also show that the signaling proteins inversin [6] and PDGFRα localize asynchronously to sister cell primary cilia and that sister cells respond asymmetrically to Shh. These results suggest that the segregation of differently aged mother centrioles, an asymmetry inherent to every animal cell division, can influence the ability of sister cells to respond to environmental signals, potentially altering the behavior or fate of one or both sister cells.

Original languageEnglish (US)
Pages (from-to)1498-1502
Number of pages5
JournalCurrent Biology
Volume19
Issue number17
DOIs
StatePublished - Sep 15 2009

Fingerprint

Centrioles
centrioles
Cilia
cilia
Cells
Platelet-Derived Growth Factor
Growth
Animals
centrosomes
Centrosome
cells
Hedgehogs
Erinaceidae
Mitosis
Cell Division
mitosis
Proteins
platelet-derived growth factor
Aptitude
Microtubules

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "Primary cilia are microtubule-based sensory organelles that play important roles in development and disease [1]. They are required for Sonic hedgehog (Shh) [2-4] and platelet-derived growth factor (PDGF) [5] signaling. Primary cilia grow from the older of the two centrioles of the centrosome, referred to as the mother centriole. In cycling cells, the cilium typically grows in G1 and is lost before mitosis, but the regulation of its growth is poorly understood. Centriole duplication at G1/S results in two centrosomes, one with an older mother centriole and one with a new mother centriole, that are segregated in mitosis. Here we report that primary cilia grow asynchronously in sister cells resulting from a mitotic division and that the sister cell receiving the older mother centriole usually grows a primary cilium first. We also show that the signaling proteins inversin [6] and PDGFRα localize asynchronously to sister cell primary cilia and that sister cells respond asymmetrically to Shh. These results suggest that the segregation of differently aged mother centrioles, an asymmetry inherent to every animal cell division, can influence the ability of sister cells to respond to environmental signals, potentially altering the behavior or fate of one or both sister cells.",
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Centriole Age Underlies Asynchronous Primary Cilium Growth in Mammalian Cells. / Anderson, Charles T.; Stearns, Tim.

In: Current Biology, Vol. 19, No. 17, 15.09.2009, p. 1498-1502.

Research output: Contribution to journalArticle

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