CEST MRI of sepsis-induced acute kidney injury

Jing Liu, Zheng Han, Guoli Chen, Yuguo Li, Jia Zhang, Jiadi Xu, Peter C.M. van Zijl, Shuixing Zhang, Guanshu Liu

Research output: Contribution to journalArticle

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Abstract

Sepsis-induced acute kidney injury (SAKI) is a major complication of kidney disease associated with increased mortality and faster progression. Therefore, the development of imaging biomarkers to detect septic AKI is of great clinical interest. In this study, we aimed to characterize the endogenous chemical exchange saturation transfer (CEST) MRI contrast in the lipopolysaccharide (LPS)-induced SAKI mouse model and to investigate the use of CEST MRI for detecting such injury. We used a SAKI mouse model that was generated by i.p. injection of 10 mg/kg LPS. The resulting kidney injury was confirmed by the elevation of serum creatinine and histology. MRI assessments were performed 24 h after LPS injection, including CEST MRI at different B1 strengths (1, 1.8 and 3 μT), T1 mapping, T2 mapping and conventional magnetization transfer contrast (MTC) MRI. The CEST MRI results were analyzed using Z-spectra, in which the normalized water signal saturation (Ssat/S0) is measured as a function of saturation frequency. Substantial decreases in CEST contrast were observed at both 3.5 and − 3.5 ppm frequency offset from water at all B1 powers, with the most significant difference obtained at a B1 of 1.8 μT. The average Ssat/S0 differences between injured and normal kidneys were 0.07 (0.55 ± 0.04 versus 0.62 ± 0.04, P = 0.0028) and 0.07 (0.50 ± 0.04 versus 0.57 ± 0.03, P = 0.0008) for 3.5 and − 3.5 ppm, respectively. In contrast, the T1 and T2 relaxation times and MTC contrast in the injured kidneys did not show a significant change compared with the normal control. Our results showed that CEST MRI is more sensitive to the pathological changes in injured kidneys than the changes in T1, T2 and MTC effect, indicating its potential clinical utility for molecular imaging of renal diseases.

Original languageEnglish (US)
Article numbere3942
JournalNMR in Biomedicine
Volume31
Issue number8
DOIs
StatePublished - Aug 2018

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Acute Kidney Injury
Magnetic resonance imaging
Sepsis
Kidney
Lipopolysaccharides
Magnetization
Injections
Molecular Imaging
Water
Wounds and Injuries
Kidney Diseases
Molecular imaging
Histology
Creatinine
Biomarkers
Relaxation time
Mortality
Serum
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

Cite this

Liu, J., Han, Z., Chen, G., Li, Y., Zhang, J., Xu, J., ... Liu, G. (2018). CEST MRI of sepsis-induced acute kidney injury. NMR in Biomedicine, 31(8), [e3942]. https://doi.org/10.1002/nbm.3942
Liu, Jing ; Han, Zheng ; Chen, Guoli ; Li, Yuguo ; Zhang, Jia ; Xu, Jiadi ; van Zijl, Peter C.M. ; Zhang, Shuixing ; Liu, Guanshu. / CEST MRI of sepsis-induced acute kidney injury. In: NMR in Biomedicine. 2018 ; Vol. 31, No. 8.
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abstract = "Sepsis-induced acute kidney injury (SAKI) is a major complication of kidney disease associated with increased mortality and faster progression. Therefore, the development of imaging biomarkers to detect septic AKI is of great clinical interest. In this study, we aimed to characterize the endogenous chemical exchange saturation transfer (CEST) MRI contrast in the lipopolysaccharide (LPS)-induced SAKI mouse model and to investigate the use of CEST MRI for detecting such injury. We used a SAKI mouse model that was generated by i.p. injection of 10 mg/kg LPS. The resulting kidney injury was confirmed by the elevation of serum creatinine and histology. MRI assessments were performed 24 h after LPS injection, including CEST MRI at different B1 strengths (1, 1.8 and 3 μT), T1 mapping, T2 mapping and conventional magnetization transfer contrast (MTC) MRI. The CEST MRI results were analyzed using Z-spectra, in which the normalized water signal saturation (Ssat/S0) is measured as a function of saturation frequency. Substantial decreases in CEST contrast were observed at both 3.5 and − 3.5 ppm frequency offset from water at all B1 powers, with the most significant difference obtained at a B1 of 1.8 μT. The average Ssat/S0 differences between injured and normal kidneys were 0.07 (0.55 ± 0.04 versus 0.62 ± 0.04, P = 0.0028) and 0.07 (0.50 ± 0.04 versus 0.57 ± 0.03, P = 0.0008) for 3.5 and − 3.5 ppm, respectively. In contrast, the T1 and T2 relaxation times and MTC contrast in the injured kidneys did not show a significant change compared with the normal control. Our results showed that CEST MRI is more sensitive to the pathological changes in injured kidneys than the changes in T1, T2 and MTC effect, indicating its potential clinical utility for molecular imaging of renal diseases.",
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Liu, J, Han, Z, Chen, G, Li, Y, Zhang, J, Xu, J, van Zijl, PCM, Zhang, S & Liu, G 2018, 'CEST MRI of sepsis-induced acute kidney injury', NMR in Biomedicine, vol. 31, no. 8, e3942. https://doi.org/10.1002/nbm.3942

CEST MRI of sepsis-induced acute kidney injury. / Liu, Jing; Han, Zheng; Chen, Guoli; Li, Yuguo; Zhang, Jia; Xu, Jiadi; van Zijl, Peter C.M.; Zhang, Shuixing; Liu, Guanshu.

In: NMR in Biomedicine, Vol. 31, No. 8, e3942, 08.2018.

Research output: Contribution to journalArticle

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Liu J, Han Z, Chen G, Li Y, Zhang J, Xu J et al. CEST MRI of sepsis-induced acute kidney injury. NMR in Biomedicine. 2018 Aug;31(8). e3942. https://doi.org/10.1002/nbm.3942