cGMP-mediated effects on the physiology of bovine and human retinal Müller (glial) cells

Shunji Kusaka, Isabelle Dabin, Colin J. Barnstable, Donald G. Puro

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

1. Whole-cell currents of freshly dissociated or cultured Müller cells from human and bovine retinas were studied using the perforated-patch and standard whole-cell recording techniques. 2. We found that internal perfusion of cGMP or external exposure to 8-bromo-cGMP activated a calcium permeable, non-selective cation current in Müller cells, the principal glial cells of the retina. In addition, the activity of calcium-activated potassium channels increased markedly. These currents were minimally affected by cAMP. 3. Molecular studies using the reverse transcription-polymerase chain reaction demonstrated that human Müller cells in culture contain transcripts closely related to the rod cyclic nucleotide-gated (CNG) channel. 4. Since guanylate cyclase is a known target for nitric oxide (NO), we tested the effect of NO donors on Müller cell currents. These agents induced currents that were qualitatively similar to those activated by cGMP. 5. Our experiments support the idea that the NO-cGMP pathway regulates the physiology of Müller cells and may play a role in integrating neuron-glia interactions in the retina.

Original languageEnglish (US)
Pages (from-to)813-824
Number of pages12
JournalJournal of Physiology
Volume497
Issue number3
DOIs
StatePublished - Dec 15 1996

All Science Journal Classification (ASJC) codes

  • Physiology

Fingerprint Dive into the research topics of 'cGMP-mediated effects on the physiology of bovine and human retinal Müller (glial) cells'. Together they form a unique fingerprint.

Cite this