cGMP-mediated effects on the physiology of bovine and human retinal Müller (glial) cells

Shunji Kusaka, Isabelle Dabin, Colin J. Barnstable, Donald G. Puro

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

1. Whole-cell currents of freshly dissociated or cultured Müller cells from human and bovine retinas were studied using the perforated-patch and standard whole-cell recording techniques. 2. We found that internal perfusion of cGMP or external exposure to 8-bromo-cGMP activated a calcium permeable, non-selective cation current in Müller cells, the principal glial cells of the retina. In addition, the activity of calcium-activated potassium channels increased markedly. These currents were minimally affected by cAMP. 3. Molecular studies using the reverse transcription-polymerase chain reaction demonstrated that human Müller cells in culture contain transcripts closely related to the rod cyclic nucleotide-gated (CNG) channel. 4. Since guanylate cyclase is a known target for nitric oxide (NO), we tested the effect of NO donors on Müller cell currents. These agents induced currents that were qualitatively similar to those activated by cGMP. 5. Our experiments support the idea that the NO-cGMP pathway regulates the physiology of Müller cells and may play a role in integrating neuron-glia interactions in the retina.

Original languageEnglish (US)
Pages (from-to)813-824
Number of pages12
JournalJournal of Physiology
Volume497
Issue number3
DOIs
StatePublished - Dec 15 1996

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Neuroglia
Retina
Nitric Oxide
Cyclic Nucleotide-Gated Cation Channels
Calcium-Activated Potassium Channels
Cell Physiological Phenomena
Nitric Oxide Donors
Guanylate Cyclase
Patch-Clamp Techniques
Reverse Transcription
Cations
Cultured Cells
Cell Culture Techniques
Perfusion
Calcium
Neurons
Polymerase Chain Reaction
8-bromocyclic GMP

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Kusaka, Shunji ; Dabin, Isabelle ; Barnstable, Colin J. ; Puro, Donald G. / cGMP-mediated effects on the physiology of bovine and human retinal Müller (glial) cells. In: Journal of Physiology. 1996 ; Vol. 497, No. 3. pp. 813-824.
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cGMP-mediated effects on the physiology of bovine and human retinal Müller (glial) cells. / Kusaka, Shunji; Dabin, Isabelle; Barnstable, Colin J.; Puro, Donald G.

In: Journal of Physiology, Vol. 497, No. 3, 15.12.1996, p. 813-824.

Research output: Contribution to journalArticle

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