TY - JOUR
T1 - Challenges and triumphs in cryo-electron tomography
AU - Hylton, Ryan K.
AU - Swulius, Matthew T.
N1 - Funding Information:
We would like to acknowledge Han Chen at the Penn State College of Medicine TEM Core for providing the image for Figure 1A, Grant Jensen at Caltech for providing images for Figure 2, and the Penn State College of Medicine Cryo-EM Core for providing images for Figure 1D, as well as figures 9C and 9D. R.H. wrote and revised the manuscript and prepared figures. M.S. conceptualized, wrote, and revised the manuscript. There are no conflicts of interest.
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/9/24
Y1 - 2021/9/24
N2 - Cryo-electron tomography has stepped fully into the spotlight. Enthusiasm is high. Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to go before declaring victory. Despite its potential, cryo-electron tomography possesses many inherent challenges. How do we image through thick cell samples, and possibly even tissue? How do we identify a protein of interest amidst the noisy, crowded environment of the cytoplasm? How do we target specific moments of a dynamic cellular process for tomographic imaging? In this review, we cover the history of cryo-electron tomography and how it came to be, roughly speaking, as well as the many approaches that have been developed to overcome its intrinsic limitations.
AB - Cryo-electron tomography has stepped fully into the spotlight. Enthusiasm is high. Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to go before declaring victory. Despite its potential, cryo-electron tomography possesses many inherent challenges. How do we image through thick cell samples, and possibly even tissue? How do we identify a protein of interest amidst the noisy, crowded environment of the cytoplasm? How do we target specific moments of a dynamic cellular process for tomographic imaging? In this review, we cover the history of cryo-electron tomography and how it came to be, roughly speaking, as well as the many approaches that have been developed to overcome its intrinsic limitations.
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U2 - 10.1016/j.isci.2021.102959
DO - 10.1016/j.isci.2021.102959
M3 - Review article
C2 - 34466785
AN - SCOPUS:85119151087
SN - 2589-0042
VL - 24
JO - iScience
JF - iScience
IS - 9
M1 - 102959
ER -