Changes in ethylene and polyamines in relation to mRNA levels of the large and small subunits of ribulose bisphosphate carboxylase/oxygenase in ozone‐stressed potato foliage

G. NAGI REDDY, R. N. ARTECA, Y. ‐R DAI, H. E. FLORES, F. B. NEGM, E. J. PELL

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Abstract

Potato plants (Solanum tuberosum L. cv‘Norland’) were subjected to acute and chronic exposures of ozone (O3). Periodically following or during the exposures, foliage of different physiological ages were sampled for tissue analysis. Measurement of the following parameters were included: ethylene, 1‐aminocyclopropane‐1‐carboxylic acid (ACC), N‐malonyl 1‐aminocyclopropane‐1‐carboxylic acid (MACC), and ACC oxidase activity, putrescine, spermidine, and spermine levels, and ornithine decarboxylase (ODC) activity, and levels of mRNA transcripts for the large and small subunits of ribulose 1,5‐bisphosphate carboxylase/oxygenase (Rubisco) (rbcL and rbcS, respectively). Ozone increased emission of ethylene and the concentration and activity of all associated metabolites and enzymes. Putrescine titre increased in response to O3 as did ODC activity. Some increases in spermidine were also detected. Ozone decreased the levels of mRNA for rbcL and rbcS, with the latter transcript exhibiting greater sensitivity. After acute exposures were terminated, the rbcL and rbcS transcript in younger leaves returned to levels of nonstressed plants; effects were less likely to be reversed in older tissue. The potential relationship between the changes in rbcL and rbcS and ethylene and polyamines are discussed in the context of O3 induction of accelerated senescence.

Original languageEnglish (US)
Pages (from-to)819-826
Number of pages8
JournalPlant, Cell & Environment
Volume16
Issue number7
DOIs
StatePublished - Sep 1993

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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