Changes in iron histochemistry after hypoxic-ischemic brain injury in the neonatal rat

Charles Palmer, Sharon L. Menzies, Rebecca L. Roberts, Geno Pavlick, James R. Connor

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Iron can contribute to hypoxic-ischemic brain damage by catalyzing the formation of free radicals. The immature brain has high iron levels and limited antioxidant defenses. The objective of this study was to describe the early alterations in nonheme iron histochemistry following a hypoxic-ischemic (HI) insult to the brain of neonatal rats. We induced a HI insult to the right cerebral hemisphere in groups of 7-day-old rats. Rats were anesthetized, then their brains were perfused and fixed at 0, 1, 4, 8, 24 hr, and 1, 2, and 3 weeks of recovery. Forty-micron-thick frozen sections were stained for iron using the intensified Perls stain. Increased iron staining was first detected within the cytoplasm of cells with pyknotic nuclei at 4 hr of recovery. Staining increased rapidly over the first 24 hr in regions of ischemic injury. By 7 days recovery, reactive glia and cortical blood vessels also stained. Increased staining in gray matter persisted at 3 weeks of recovery, whereas white matter tracts had fewer iron-positive cells compared to normal. The early increase in iron staining could be caused by an accumulation of iron posthypoxic-ischemic injury or a change in iron from nonstainable heme iron to stainable nonheme iron. Regardless of the source, our results indicate that there is an increase in iron available to promote oxidant stress in the neonatal rat brain following hypoxia-ischemia.

Original languageEnglish (US)
Pages (from-to)60-71
Number of pages12
JournalJournal of Neuroscience Research
Volume56
Issue number1
DOIs
StatePublished - Apr 1 1999

Fingerprint

Brain Injuries
Iron
Staining and Labeling
Brain
Brain Hypoxia-Ischemia
Brain Hypoxia
Wounds and Injuries
Cerebrum
Frozen Sections
Heme
Oxidants
Neuroglia
Free Radicals
Blood Vessels
Cytoplasm
Coloring Agents
Antioxidants

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

Palmer, Charles ; Menzies, Sharon L. ; Roberts, Rebecca L. ; Pavlick, Geno ; Connor, James R. / Changes in iron histochemistry after hypoxic-ischemic brain injury in the neonatal rat. In: Journal of Neuroscience Research. 1999 ; Vol. 56, No. 1. pp. 60-71.
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Changes in iron histochemistry after hypoxic-ischemic brain injury in the neonatal rat. / Palmer, Charles; Menzies, Sharon L.; Roberts, Rebecca L.; Pavlick, Geno; Connor, James R.

In: Journal of Neuroscience Research, Vol. 56, No. 1, 01.04.1999, p. 60-71.

Research output: Contribution to journalArticle

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