Changes in proteomic profiles in different prostate lobes of male rats throughout growth and development and aging stages of the life span

Arunangshu Das, James D. Bortner, Cesar Aliaga, Aaron Baker, Anne Stanley, Bruce Stanley, Matthew G. Kaag, John Richie, Karam El-Bayoumy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

BACKGROUND Aging-related changes in important cellular pathways in the prostate may promote a permissive environment for an increased risk for prostatic disease development such as prostate cancer. Our objectives were to examine for such changes, by systematically determining the effects of growth and development and aging on proteomic profiles in different lobes of the rat prostate. METHODS Prostate lobes (dorsolateral lobe, DL and ventral lobe, VL) were obtained from male Fisher rats of various ages representing young (4 months), mature (12 months), old (18 months), and very old (24 months). Differentially expressed proteins between age groups in each lobe were identified using a proteomic approach, isobaric Tags for Relative and Absolute Quantitation (iTRAQ). Select changes in the DL and VL were verified by immunoblot analysis. RESULTS iTRAQ identified 317 proteins with high confidence. iTRAQ discovered 12 and 6 proteins significantly modulated in response to growth and development in the DL and VL, respectively, and 42 and 29 proteins significantly modulated in response to aging in the DL and VL, respectively. Proteins modulated during growth and development in the DL and VL are involved in a variety of biological processes including cell communication and development, whereas proteins modulated during aging were predominantly related to antioxidant activity and immunity. Immunoblot analysis verified age-related changes for α-1 antitrypsin, annexin A1, hypoxia up-regulated protein 1, and 78 kDa glucose-regulated protein. CONCLUSIONS Aging results in changes in numerous prostatic proteins and pathways which are mainly linked to inflammation and may lead to prostatic disease development. Prostate 73: 363-375, 2013. © 2012 Wiley Periodicals, Inc.

Original languageEnglish (US)
Pages (from-to)363-375
Number of pages13
JournalProstate
Volume73
Issue number4
DOIs
StatePublished - Mar 1 2013

Fingerprint

Growth and Development
Proteomics
Prostate
Proteins
Prostatic Diseases
Annexin A1
Biological Phenomena
Cell Communication
Immunity
Prostatic Neoplasms
Age Groups
Antioxidants
Inflammation

All Science Journal Classification (ASJC) codes

  • Oncology
  • Urology

Cite this

@article{3d2e8c0ac95041009c880442bae3c12d,
title = "Changes in proteomic profiles in different prostate lobes of male rats throughout growth and development and aging stages of the life span",
abstract = "BACKGROUND Aging-related changes in important cellular pathways in the prostate may promote a permissive environment for an increased risk for prostatic disease development such as prostate cancer. Our objectives were to examine for such changes, by systematically determining the effects of growth and development and aging on proteomic profiles in different lobes of the rat prostate. METHODS Prostate lobes (dorsolateral lobe, DL and ventral lobe, VL) were obtained from male Fisher rats of various ages representing young (4 months), mature (12 months), old (18 months), and very old (24 months). Differentially expressed proteins between age groups in each lobe were identified using a proteomic approach, isobaric Tags for Relative and Absolute Quantitation (iTRAQ). Select changes in the DL and VL were verified by immunoblot analysis. RESULTS iTRAQ identified 317 proteins with high confidence. iTRAQ discovered 12 and 6 proteins significantly modulated in response to growth and development in the DL and VL, respectively, and 42 and 29 proteins significantly modulated in response to aging in the DL and VL, respectively. Proteins modulated during growth and development in the DL and VL are involved in a variety of biological processes including cell communication and development, whereas proteins modulated during aging were predominantly related to antioxidant activity and immunity. Immunoblot analysis verified age-related changes for α-1 antitrypsin, annexin A1, hypoxia up-regulated protein 1, and 78 kDa glucose-regulated protein. CONCLUSIONS Aging results in changes in numerous prostatic proteins and pathways which are mainly linked to inflammation and may lead to prostatic disease development. Prostate 73: 363-375, 2013. {\circledC} 2012 Wiley Periodicals, Inc.",
author = "Arunangshu Das and Bortner, {James D.} and Cesar Aliaga and Aaron Baker and Anne Stanley and Bruce Stanley and Kaag, {Matthew G.} and John Richie and Karam El-Bayoumy",
year = "2013",
month = "3",
day = "1",
doi = "10.1002/pros.22576",
language = "English (US)",
volume = "73",
pages = "363--375",
journal = "Prostate",
issn = "0270-4137",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Changes in proteomic profiles in different prostate lobes of male rats throughout growth and development and aging stages of the life span

AU - Das, Arunangshu

AU - Bortner, James D.

AU - Aliaga, Cesar

AU - Baker, Aaron

AU - Stanley, Anne

AU - Stanley, Bruce

AU - Kaag, Matthew G.

AU - Richie, John

AU - El-Bayoumy, Karam

PY - 2013/3/1

Y1 - 2013/3/1

N2 - BACKGROUND Aging-related changes in important cellular pathways in the prostate may promote a permissive environment for an increased risk for prostatic disease development such as prostate cancer. Our objectives were to examine for such changes, by systematically determining the effects of growth and development and aging on proteomic profiles in different lobes of the rat prostate. METHODS Prostate lobes (dorsolateral lobe, DL and ventral lobe, VL) were obtained from male Fisher rats of various ages representing young (4 months), mature (12 months), old (18 months), and very old (24 months). Differentially expressed proteins between age groups in each lobe were identified using a proteomic approach, isobaric Tags for Relative and Absolute Quantitation (iTRAQ). Select changes in the DL and VL were verified by immunoblot analysis. RESULTS iTRAQ identified 317 proteins with high confidence. iTRAQ discovered 12 and 6 proteins significantly modulated in response to growth and development in the DL and VL, respectively, and 42 and 29 proteins significantly modulated in response to aging in the DL and VL, respectively. Proteins modulated during growth and development in the DL and VL are involved in a variety of biological processes including cell communication and development, whereas proteins modulated during aging were predominantly related to antioxidant activity and immunity. Immunoblot analysis verified age-related changes for α-1 antitrypsin, annexin A1, hypoxia up-regulated protein 1, and 78 kDa glucose-regulated protein. CONCLUSIONS Aging results in changes in numerous prostatic proteins and pathways which are mainly linked to inflammation and may lead to prostatic disease development. Prostate 73: 363-375, 2013. © 2012 Wiley Periodicals, Inc.

AB - BACKGROUND Aging-related changes in important cellular pathways in the prostate may promote a permissive environment for an increased risk for prostatic disease development such as prostate cancer. Our objectives were to examine for such changes, by systematically determining the effects of growth and development and aging on proteomic profiles in different lobes of the rat prostate. METHODS Prostate lobes (dorsolateral lobe, DL and ventral lobe, VL) were obtained from male Fisher rats of various ages representing young (4 months), mature (12 months), old (18 months), and very old (24 months). Differentially expressed proteins between age groups in each lobe were identified using a proteomic approach, isobaric Tags for Relative and Absolute Quantitation (iTRAQ). Select changes in the DL and VL were verified by immunoblot analysis. RESULTS iTRAQ identified 317 proteins with high confidence. iTRAQ discovered 12 and 6 proteins significantly modulated in response to growth and development in the DL and VL, respectively, and 42 and 29 proteins significantly modulated in response to aging in the DL and VL, respectively. Proteins modulated during growth and development in the DL and VL are involved in a variety of biological processes including cell communication and development, whereas proteins modulated during aging were predominantly related to antioxidant activity and immunity. Immunoblot analysis verified age-related changes for α-1 antitrypsin, annexin A1, hypoxia up-regulated protein 1, and 78 kDa glucose-regulated protein. CONCLUSIONS Aging results in changes in numerous prostatic proteins and pathways which are mainly linked to inflammation and may lead to prostatic disease development. Prostate 73: 363-375, 2013. © 2012 Wiley Periodicals, Inc.

UR - http://www.scopus.com/inward/record.url?scp=84872914177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872914177&partnerID=8YFLogxK

U2 - 10.1002/pros.22576

DO - 10.1002/pros.22576

M3 - Article

C2 - 22911278

AN - SCOPUS:84872914177

VL - 73

SP - 363

EP - 375

JO - Prostate

JF - Prostate

SN - 0270-4137

IS - 4

ER -