Changes in Retinal N-Acylethanolamines and their Oxylipin Derivatives During the Development of Visual Impairment in a Mouse Model for Glaucoma

Christa L. Montgomery, Jantana Keereetaweep, Heather M. Johnson, Stephanie L. Grillo, Kent D. Chapman, Peter Koulen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Neurons are especially susceptible to oxidative damage, which is increasingly implicated in neurodegenerative disease. Certain N-acylethanolamines (NAEs) have been shown to protect neurons from oxidative stress. Since glaucoma may be considered a neurodegenerative disorder and the survival of retinal neurons could also be influenced by N-acylethanolamines, our goal was to quantify changes in certain N-acylethanolamine species and their oxylipin derivatives in the retina of a mouse model for glaucoma. We also sought to identify relationships between these and parameters of glaucoma disease development, specifically intraocular pressure, visual acuity, and contrast sensitivity. Five N-acylethanolamine species and three NAE oxylipin derivatives were quantified in retina from young and aged DBA/2Crl mice. N-Acylethanolamines and NAE-oxylipins in retinal extracts were quantified against deuterated standards by isotope dilution gas chromatography–mass spectrometry. Levels (nmol/g dry weight) of N-arachidonoylethanolamine (anandamide; NAE 20:4) were significantly (p = 0.008) decreased in aged (2.875 ± 0.6702) compared to young animals (5.175 ± 0.971). Conversely, the anandamide oxylipin, 15(S)-HETE ethanolamide (15(S)-HETE EA), was significantly (p = 0.042) increased in aged (0.063 ± 0.009) compared to young animals (0.039 ± 0.011). Enzymatic depletion of the anandamide pool by 15-lipoxygenase and consequent accumulation of 15(S)-HETE ethanolamine may contribute to decreased visual function in glaucomatous mice. Since N-acylethanolamines effectively attenuate glaucoma pathogenesis and associated visual impairment, our data provides additional rationale and novel targets for glaucoma therapies.

Original languageEnglish (US)
Pages (from-to)857-866
Number of pages10
JournalLipids
Volume51
Issue number7
DOIs
StatePublished - Jul 1 2016

Fingerprint

Oxylipins
Vision Disorders
Glaucoma
Derivatives
Hydroxyeicosatetraenoic Acids
Neurons
Neurodegenerative Diseases
Retina
Animals
Arachidonate 15-Lipoxygenase
Neurodegenerative diseases
Retinal Neurons
N-acylethanolamines
Inbred DBA Mouse
Contrast Sensitivity
Ethanolamine
Oxidative stress
Intraocular Pressure
Isotopes
Spectrometry

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Montgomery, Christa L. ; Keereetaweep, Jantana ; Johnson, Heather M. ; Grillo, Stephanie L. ; Chapman, Kent D. ; Koulen, Peter. / Changes in Retinal N-Acylethanolamines and their Oxylipin Derivatives During the Development of Visual Impairment in a Mouse Model for Glaucoma. In: Lipids. 2016 ; Vol. 51, No. 7. pp. 857-866.
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Changes in Retinal N-Acylethanolamines and their Oxylipin Derivatives During the Development of Visual Impairment in a Mouse Model for Glaucoma. / Montgomery, Christa L.; Keereetaweep, Jantana; Johnson, Heather M.; Grillo, Stephanie L.; Chapman, Kent D.; Koulen, Peter.

In: Lipids, Vol. 51, No. 7, 01.07.2016, p. 857-866.

Research output: Contribution to journalArticle

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