Changes in root cap pH are required for the gravity response of the Arabidopsis root

J. M. Fasano, S. J. Swanson, E. B. Blancaflor, P. E. Dowd, T. H. Kao, S. Gilroy

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

Although the columella cells of the root cap have been identified as the site of gravity perception, the cellular events that mediate gravity signaling remain poorly understood. To determine if cytoplasmic and/or wall pH mediates the initial stages of root gravitropism, we combined a novel cell wall pH sensor (a cellulose binding domain pepfide-Oregon green conjugate) and a cytoplasmic pH sensor (plants expressing pH-sensitive green fluorescent protein) to monitor pH dynamics throughout the graviresponding Arabidopsis root. The root cap apoplast acidified from pH 5.5 to 4.5 within 2 min of gravistimulation. Concomitantly, cytoplasmic pH increased in columella cells from 7.2 to 7.6 but was unchanged elsewhere in the root. These changes in cap pH preceded detectable tropic growth or growth-related pH changes in the elongation zone cell wall by 10 min. Altering the gravity-related columella cytoplasmic pH shift with caged protons delayed the gravitropic response. Together, these results suggest that alterations in root cap pH likely are involved in the initial events that mediate root gravity perception or signal transduction.

Original languageEnglish (US)
Pages (from-to)907-921
Number of pages15
JournalPlant Cell
Volume13
Issue number4
DOIs
StatePublished - May 8 2001

Fingerprint

root cap
Gravitation
Arabidopsis
gravity
Gravity Sensing
Cell Wall
Gravitropism
cell walls
gravitropism
apoplast
Growth
Green Fluorescent Proteins
green fluorescent protein
Cellulose
protons
signal transduction
Protons
Signal Transduction
tropics
cellulose

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Fasano, J. M., Swanson, S. J., Blancaflor, E. B., Dowd, P. E., Kao, T. H., & Gilroy, S. (2001). Changes in root cap pH are required for the gravity response of the Arabidopsis root. Plant Cell, 13(4), 907-921. https://doi.org/10.1105/tpc.13.4.907
Fasano, J. M. ; Swanson, S. J. ; Blancaflor, E. B. ; Dowd, P. E. ; Kao, T. H. ; Gilroy, S. / Changes in root cap pH are required for the gravity response of the Arabidopsis root. In: Plant Cell. 2001 ; Vol. 13, No. 4. pp. 907-921.
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Fasano, JM, Swanson, SJ, Blancaflor, EB, Dowd, PE, Kao, TH & Gilroy, S 2001, 'Changes in root cap pH are required for the gravity response of the Arabidopsis root', Plant Cell, vol. 13, no. 4, pp. 907-921. https://doi.org/10.1105/tpc.13.4.907

Changes in root cap pH are required for the gravity response of the Arabidopsis root. / Fasano, J. M.; Swanson, S. J.; Blancaflor, E. B.; Dowd, P. E.; Kao, T. H.; Gilroy, S.

In: Plant Cell, Vol. 13, No. 4, 08.05.2001, p. 907-921.

Research output: Contribution to journalArticle

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