Changes in the relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels

Richard Cyr, Michael Sotak, Mauricio M. Bustos, Mark Guiltinan, Donald E. Fosket

Research output: Contribution to journalArticle

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Abstract

The relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels varies depending upon the electrophoretic methods used to separate them. When reduced and alkylated rat and carrot tubulin heterodimers were separated by one method, the α-tubulin subunits, identified by means of a highly specific antibody, migrated more slowly than the β-tubulin subunits. However, when separated by another method, the carrot α-subunit migrated more rapidly than its β-subunit, while the relative mobility of rat brain tubulin subunits was unchanged. The two gel systems differ principally in the pH of the separating gel during electrophoresis, suggesting that pH markedly influences the interaction of SDS with the plant, but not the vertebrate α-tubulin.

Original languageEnglish (US)
Pages (from-to)28-34
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume914
Issue number1
DOIs
StatePublished - Jul 24 1987

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Electrophoretic mobility
Tubulin
Daucus carota
Rats
Gels
Electrophoresis
Vertebrates
polyacrylamide gels
Brain
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

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title = "Changes in the relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels",
abstract = "The relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels varies depending upon the electrophoretic methods used to separate them. When reduced and alkylated rat and carrot tubulin heterodimers were separated by one method, the α-tubulin subunits, identified by means of a highly specific antibody, migrated more slowly than the β-tubulin subunits. However, when separated by another method, the carrot α-subunit migrated more rapidly than its β-subunit, while the relative mobility of rat brain tubulin subunits was unchanged. The two gel systems differ principally in the pH of the separating gel during electrophoresis, suggesting that pH markedly influences the interaction of SDS with the plant, but not the vertebrate α-tubulin.",
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Changes in the relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels. / Cyr, Richard; Sotak, Michael; Bustos, Mauricio M.; Guiltinan, Mark; Fosket, Donald E.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 914, No. 1, 24.07.1987, p. 28-34.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Changes in the relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels

AU - Cyr, Richard

AU - Sotak, Michael

AU - Bustos, Mauricio M.

AU - Guiltinan, Mark

AU - Fosket, Donald E.

PY - 1987/7/24

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AB - The relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels varies depending upon the electrophoretic methods used to separate them. When reduced and alkylated rat and carrot tubulin heterodimers were separated by one method, the α-tubulin subunits, identified by means of a highly specific antibody, migrated more slowly than the β-tubulin subunits. However, when separated by another method, the carrot α-subunit migrated more rapidly than its β-subunit, while the relative mobility of rat brain tubulin subunits was unchanged. The two gel systems differ principally in the pH of the separating gel during electrophoresis, suggesting that pH markedly influences the interaction of SDS with the plant, but not the vertebrate α-tubulin.

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