Chapter 4

Directed differentiation of human pluripotent stem cells for therapeutic applications

Lauren N. Randolph, Evan B. Witmer, Xiaojun Lian

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, are a potentially inexhaustible supply of human cells because they can be propagated indefinitely while still retaining the capacity to differentiate into all somatic cell types. Hurdles facing utilization of hPSCs in regenerative medicine include lack of reliable and efficient methods to differentiate hPSCs into disease related cell lineages, including neurons, cardiac muscle cells and pancreatic beta cells. During differentiation, hPSCs make a number of developmental decisions to generate first the three germ layers (ectoderm, mesoderm and endoderm), followed by subsequent decisions to generate all of the body's mature tissues. These differentiation processes often involve a limited set of developmental cell signaling pathways, which can be modulated by growth factors and small molecules. Therefore, common methods of differentiating hPSCs to therapeutic applicable cell lineages require application of specific combination of growth factors and/or small molecules at distinct differentiation stages. This chapter discusses recent progress in the production of therapeutically applicable cells via directed differentiation of hPSCs.

Original languageEnglish (US)
Title of host publicationFrontiers in Nanobiomedical Research
PublisherWorld Scientific Publishing Co. Pte Ltd
Pages75-100
Number of pages26
Volume10
StatePublished - 2018

Publication series

NameFrontiers in Nanobiomedical Research
Volume10
ISSN (Print)2251-3965

Fingerprint

Pluripotent Stem Cells
Stem cells
Cell Lineage
Therapeutics
Intercellular Signaling Peptides and Proteins
Cells
Germ Layers
Induced Pluripotent Stem Cells
Endoderm
Ectoderm
Regenerative Medicine
Cell signaling
Insulin-Secreting Cells
Mesoderm
Molecules
Cardiac Myocytes
Neurons
Muscle
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biotechnology
  • Cancer Research
  • Clinical Biochemistry
  • Molecular Biology
  • Medicine (miscellaneous)

Cite this

Randolph, L. N., Witmer, E. B., & Lian, X. (2018). Chapter 4: Directed differentiation of human pluripotent stem cells for therapeutic applications. In Frontiers in Nanobiomedical Research (Vol. 10, pp. 75-100). (Frontiers in Nanobiomedical Research; Vol. 10). World Scientific Publishing Co. Pte Ltd.
Randolph, Lauren N. ; Witmer, Evan B. ; Lian, Xiaojun. / Chapter 4 : Directed differentiation of human pluripotent stem cells for therapeutic applications. Frontiers in Nanobiomedical Research. Vol. 10 World Scientific Publishing Co. Pte Ltd, 2018. pp. 75-100 (Frontiers in Nanobiomedical Research).
@inbook{82125b8153f3483d8b15dfacb7dcc6d0,
title = "Chapter 4: Directed differentiation of human pluripotent stem cells for therapeutic applications",
abstract = "Human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, are a potentially inexhaustible supply of human cells because they can be propagated indefinitely while still retaining the capacity to differentiate into all somatic cell types. Hurdles facing utilization of hPSCs in regenerative medicine include lack of reliable and efficient methods to differentiate hPSCs into disease related cell lineages, including neurons, cardiac muscle cells and pancreatic beta cells. During differentiation, hPSCs make a number of developmental decisions to generate first the three germ layers (ectoderm, mesoderm and endoderm), followed by subsequent decisions to generate all of the body's mature tissues. These differentiation processes often involve a limited set of developmental cell signaling pathways, which can be modulated by growth factors and small molecules. Therefore, common methods of differentiating hPSCs to therapeutic applicable cell lineages require application of specific combination of growth factors and/or small molecules at distinct differentiation stages. This chapter discusses recent progress in the production of therapeutically applicable cells via directed differentiation of hPSCs.",
author = "Randolph, {Lauren N.} and Witmer, {Evan B.} and Xiaojun Lian",
year = "2018",
language = "English (US)",
volume = "10",
series = "Frontiers in Nanobiomedical Research",
publisher = "World Scientific Publishing Co. Pte Ltd",
pages = "75--100",
booktitle = "Frontiers in Nanobiomedical Research",
address = "Singapore",

}

Randolph, LN, Witmer, EB & Lian, X 2018, Chapter 4: Directed differentiation of human pluripotent stem cells for therapeutic applications. in Frontiers in Nanobiomedical Research. vol. 10, Frontiers in Nanobiomedical Research, vol. 10, World Scientific Publishing Co. Pte Ltd, pp. 75-100.

Chapter 4 : Directed differentiation of human pluripotent stem cells for therapeutic applications. / Randolph, Lauren N.; Witmer, Evan B.; Lian, Xiaojun.

Frontiers in Nanobiomedical Research. Vol. 10 World Scientific Publishing Co. Pte Ltd, 2018. p. 75-100 (Frontiers in Nanobiomedical Research; Vol. 10).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Chapter 4

T2 - Directed differentiation of human pluripotent stem cells for therapeutic applications

AU - Randolph, Lauren N.

AU - Witmer, Evan B.

AU - Lian, Xiaojun

PY - 2018

Y1 - 2018

N2 - Human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, are a potentially inexhaustible supply of human cells because they can be propagated indefinitely while still retaining the capacity to differentiate into all somatic cell types. Hurdles facing utilization of hPSCs in regenerative medicine include lack of reliable and efficient methods to differentiate hPSCs into disease related cell lineages, including neurons, cardiac muscle cells and pancreatic beta cells. During differentiation, hPSCs make a number of developmental decisions to generate first the three germ layers (ectoderm, mesoderm and endoderm), followed by subsequent decisions to generate all of the body's mature tissues. These differentiation processes often involve a limited set of developmental cell signaling pathways, which can be modulated by growth factors and small molecules. Therefore, common methods of differentiating hPSCs to therapeutic applicable cell lineages require application of specific combination of growth factors and/or small molecules at distinct differentiation stages. This chapter discusses recent progress in the production of therapeutically applicable cells via directed differentiation of hPSCs.

AB - Human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, are a potentially inexhaustible supply of human cells because they can be propagated indefinitely while still retaining the capacity to differentiate into all somatic cell types. Hurdles facing utilization of hPSCs in regenerative medicine include lack of reliable and efficient methods to differentiate hPSCs into disease related cell lineages, including neurons, cardiac muscle cells and pancreatic beta cells. During differentiation, hPSCs make a number of developmental decisions to generate first the three germ layers (ectoderm, mesoderm and endoderm), followed by subsequent decisions to generate all of the body's mature tissues. These differentiation processes often involve a limited set of developmental cell signaling pathways, which can be modulated by growth factors and small molecules. Therefore, common methods of differentiating hPSCs to therapeutic applicable cell lineages require application of specific combination of growth factors and/or small molecules at distinct differentiation stages. This chapter discusses recent progress in the production of therapeutically applicable cells via directed differentiation of hPSCs.

UR - http://www.scopus.com/inward/record.url?scp=85032183882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032183882&partnerID=8YFLogxK

M3 - Chapter

VL - 10

T3 - Frontiers in Nanobiomedical Research

SP - 75

EP - 100

BT - Frontiers in Nanobiomedical Research

PB - World Scientific Publishing Co. Pte Ltd

ER -

Randolph LN, Witmer EB, Lian X. Chapter 4: Directed differentiation of human pluripotent stem cells for therapeutic applications. In Frontiers in Nanobiomedical Research. Vol. 10. World Scientific Publishing Co. Pte Ltd. 2018. p. 75-100. (Frontiers in Nanobiomedical Research).