Characteristics of basolateral Cl- transport by gastric surface epithelium in Necturus antral mucosa

David Soybel, M. B E Davis, L. Y. Cheung

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Conventional and ion-selective microelectrodes were used to characterize transport of Cl- across the basolateral cell membranes of gastric surface epithelium in isolated preparations of gastric antrum of Necturus. Conventional, voltage-sensing electrodes were used to evaluate changes in membrane potentials and resistances during removal of Cl- from the nutrient perfusate. Liquid ion exchanger Cl--selective microelectrodes were constructed and validated to measure intracellular Cl- activity (a(Cl)/(i)). Our data indicate that 1) a(Cl)/(i) (range 12-25 mM) is close to that predicted if Cl- is distributed across the cell membranes by electrochemical equilibrium, 2) a(Cl)/(i) is not influenced by changes in luminal Cl- content but is susceptible to changes in nutrient Cl- content, 3) Cl- conductances cannot be detected in the basolateral membrane and changes in membrane potentials do not influence a(Cl)/(i), and 4) Cl- accumulation across the basolateral membrane depends on Na+ and the level of [K+] in the nutrient solution. Inhibition of K+-dependent Cl- accumulation, in the absence of nutrient Na+ or in the presence of the inhibitor bumetanide, was demonstrated. These findings suggest that basolateral Na+-K+-Cl- cotransport is important in regulating cell Cl- levels in surface cells of the gastric antrum in Necturus.

Original languageEnglish (US)
Pages (from-to)G910-G920
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume264
Issue number5 27-5
DOIs
StatePublished - Jan 1 1993

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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