Characteristics of estrogen-2/4-hydroxylase of porcine ovarian follicles: Influence of steroidal and non-steroidal agents on the activity of the enzyme In vitro

Judith S. Mondschein, James M. Hammond, Judith Weisz

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Abstract

The conversion of [3H]estradiol to 2-hydroxyestradiol (2-OH-E2) by homogenates of porcine ovarian follicles was assayed in vitro in the presence and absence of 10 and 100 μM concentrations of the following potential substrates or inhibitors of estrogen- 2 4-hydroxylase (E- 2 4-H): (1) estrogens; estrone (E1), estriol (E3) and 17α-estradiol (17α-E2), (2) catecholestrogens; 2-hydroxyestradiol (2-OH-E2), 4-hydroxyestradiol (4-OH-E2) and 2-hydroxyestrone (2-OH-E1); (3) 2-methoxyestradiol (2-MeO-E2); (4) halogenated estrogens; 2-bromoestradiol, (2-Bromo-E2) 4-bromoestradiol and 2,4-dibromoestradiol; (5) androgens; testosterone (T), dihydrotestosterone (DHT) and androstenedione; (6) progesterone; (7) epinephrine; (8) inhibitors of steroid aromatase; aminoglutethimide and 4-hydroxyandrostenedione and (9) SKF 525A, an inhibitor of cytochrome P-450. Progesterone and 2-Bromo-E2 were the two most effective inhibitors (2-OH-E2 formation =4 and 5% of control at 100 μM and 29.6 and 17.4% at 10 μM of progesterone and 2-Bromo-E2, respectively). 2-MeO-E2 at 100 μM was nearly as effective as progesterone in inhibiting E- 2 4-H activity but only caused about 50% inhibition at 10 μM. The three catecholestrogens reduced 2-OH-E2 formation to about the same degree (21-23% of control at 100 μM). The 2,4-dibromo-E2 was equipotent with the catecholestrogens while 4-bromo-E2 was about half as effective. The phenolic estrogens, potential substrates for the enzyme, reduced 2-OH-E2 formation to different degrees, with E3 being the most effective. Among the androgens, DHT was almost as effective an inhibitor as the catecholestrogens, T was about half as effective while androstenedione had no effect. Epinephrine and the two inhibitors of aromatase did not inhibit E- 2 4-H activity. SKF 525A inhibited E- 2 4-H activity but with a potency only about 1 10th that reported for liver.

Original languageEnglish (US)
Pages (from-to)121-124
Number of pages4
JournalJournal of Steroid Biochemistry
Volume26
Issue number1
DOIs
StatePublished - Jan 1987

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Ovarian Follicle
Mixed Function Oxygenases
Catechol Estrogens
Estrogens
Swine
Enzymes
Progesterone
Proadifen
Aromatase Inhibitors
Androstenedione
Dihydrotestosterone
Androgens
Epinephrine
Estradiol
Aminoglutethimide
Estriol
Aromatase
Estrone
Substrates
estrogen 2-hydroxylase

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

Cite this

@article{539a84f5a9094197ae3ea99f015fce66,
title = "Characteristics of estrogen-2/4-hydroxylase of porcine ovarian follicles: Influence of steroidal and non-steroidal agents on the activity of the enzyme In vitro",
abstract = "The conversion of [3H]estradiol to 2-hydroxyestradiol (2-OH-E2) by homogenates of porcine ovarian follicles was assayed in vitro in the presence and absence of 10 and 100 μM concentrations of the following potential substrates or inhibitors of estrogen- 2 4-hydroxylase (E- 2 4-H): (1) estrogens; estrone (E1), estriol (E3) and 17α-estradiol (17α-E2), (2) catecholestrogens; 2-hydroxyestradiol (2-OH-E2), 4-hydroxyestradiol (4-OH-E2) and 2-hydroxyestrone (2-OH-E1); (3) 2-methoxyestradiol (2-MeO-E2); (4) halogenated estrogens; 2-bromoestradiol, (2-Bromo-E2) 4-bromoestradiol and 2,4-dibromoestradiol; (5) androgens; testosterone (T), dihydrotestosterone (DHT) and androstenedione; (6) progesterone; (7) epinephrine; (8) inhibitors of steroid aromatase; aminoglutethimide and 4-hydroxyandrostenedione and (9) SKF 525A, an inhibitor of cytochrome P-450. Progesterone and 2-Bromo-E2 were the two most effective inhibitors (2-OH-E2 formation =4 and 5{\%} of control at 100 μM and 29.6 and 17.4{\%} at 10 μM of progesterone and 2-Bromo-E2, respectively). 2-MeO-E2 at 100 μM was nearly as effective as progesterone in inhibiting E- 2 4-H activity but only caused about 50{\%} inhibition at 10 μM. The three catecholestrogens reduced 2-OH-E2 formation to about the same degree (21-23{\%} of control at 100 μM). The 2,4-dibromo-E2 was equipotent with the catecholestrogens while 4-bromo-E2 was about half as effective. The phenolic estrogens, potential substrates for the enzyme, reduced 2-OH-E2 formation to different degrees, with E3 being the most effective. Among the androgens, DHT was almost as effective an inhibitor as the catecholestrogens, T was about half as effective while androstenedione had no effect. Epinephrine and the two inhibitors of aromatase did not inhibit E- 2 4-H activity. SKF 525A inhibited E- 2 4-H activity but with a potency only about 1 10th that reported for liver.",
author = "Mondschein, {Judith S.} and Hammond, {James M.} and Judith Weisz",
year = "1987",
month = "1",
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}

Characteristics of estrogen-2/4-hydroxylase of porcine ovarian follicles : Influence of steroidal and non-steroidal agents on the activity of the enzyme In vitro. / Mondschein, Judith S.; Hammond, James M.; Weisz, Judith.

In: Journal of Steroid Biochemistry, Vol. 26, No. 1, 01.1987, p. 121-124.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characteristics of estrogen-2/4-hydroxylase of porcine ovarian follicles

T2 - Influence of steroidal and non-steroidal agents on the activity of the enzyme In vitro

AU - Mondschein, Judith S.

AU - Hammond, James M.

AU - Weisz, Judith

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N2 - The conversion of [3H]estradiol to 2-hydroxyestradiol (2-OH-E2) by homogenates of porcine ovarian follicles was assayed in vitro in the presence and absence of 10 and 100 μM concentrations of the following potential substrates or inhibitors of estrogen- 2 4-hydroxylase (E- 2 4-H): (1) estrogens; estrone (E1), estriol (E3) and 17α-estradiol (17α-E2), (2) catecholestrogens; 2-hydroxyestradiol (2-OH-E2), 4-hydroxyestradiol (4-OH-E2) and 2-hydroxyestrone (2-OH-E1); (3) 2-methoxyestradiol (2-MeO-E2); (4) halogenated estrogens; 2-bromoestradiol, (2-Bromo-E2) 4-bromoestradiol and 2,4-dibromoestradiol; (5) androgens; testosterone (T), dihydrotestosterone (DHT) and androstenedione; (6) progesterone; (7) epinephrine; (8) inhibitors of steroid aromatase; aminoglutethimide and 4-hydroxyandrostenedione and (9) SKF 525A, an inhibitor of cytochrome P-450. Progesterone and 2-Bromo-E2 were the two most effective inhibitors (2-OH-E2 formation =4 and 5% of control at 100 μM and 29.6 and 17.4% at 10 μM of progesterone and 2-Bromo-E2, respectively). 2-MeO-E2 at 100 μM was nearly as effective as progesterone in inhibiting E- 2 4-H activity but only caused about 50% inhibition at 10 μM. The three catecholestrogens reduced 2-OH-E2 formation to about the same degree (21-23% of control at 100 μM). The 2,4-dibromo-E2 was equipotent with the catecholestrogens while 4-bromo-E2 was about half as effective. The phenolic estrogens, potential substrates for the enzyme, reduced 2-OH-E2 formation to different degrees, with E3 being the most effective. Among the androgens, DHT was almost as effective an inhibitor as the catecholestrogens, T was about half as effective while androstenedione had no effect. Epinephrine and the two inhibitors of aromatase did not inhibit E- 2 4-H activity. SKF 525A inhibited E- 2 4-H activity but with a potency only about 1 10th that reported for liver.

AB - The conversion of [3H]estradiol to 2-hydroxyestradiol (2-OH-E2) by homogenates of porcine ovarian follicles was assayed in vitro in the presence and absence of 10 and 100 μM concentrations of the following potential substrates or inhibitors of estrogen- 2 4-hydroxylase (E- 2 4-H): (1) estrogens; estrone (E1), estriol (E3) and 17α-estradiol (17α-E2), (2) catecholestrogens; 2-hydroxyestradiol (2-OH-E2), 4-hydroxyestradiol (4-OH-E2) and 2-hydroxyestrone (2-OH-E1); (3) 2-methoxyestradiol (2-MeO-E2); (4) halogenated estrogens; 2-bromoestradiol, (2-Bromo-E2) 4-bromoestradiol and 2,4-dibromoestradiol; (5) androgens; testosterone (T), dihydrotestosterone (DHT) and androstenedione; (6) progesterone; (7) epinephrine; (8) inhibitors of steroid aromatase; aminoglutethimide and 4-hydroxyandrostenedione and (9) SKF 525A, an inhibitor of cytochrome P-450. Progesterone and 2-Bromo-E2 were the two most effective inhibitors (2-OH-E2 formation =4 and 5% of control at 100 μM and 29.6 and 17.4% at 10 μM of progesterone and 2-Bromo-E2, respectively). 2-MeO-E2 at 100 μM was nearly as effective as progesterone in inhibiting E- 2 4-H activity but only caused about 50% inhibition at 10 μM. The three catecholestrogens reduced 2-OH-E2 formation to about the same degree (21-23% of control at 100 μM). The 2,4-dibromo-E2 was equipotent with the catecholestrogens while 4-bromo-E2 was about half as effective. The phenolic estrogens, potential substrates for the enzyme, reduced 2-OH-E2 formation to different degrees, with E3 being the most effective. Among the androgens, DHT was almost as effective an inhibitor as the catecholestrogens, T was about half as effective while androstenedione had no effect. Epinephrine and the two inhibitors of aromatase did not inhibit E- 2 4-H activity. SKF 525A inhibited E- 2 4-H activity but with a potency only about 1 10th that reported for liver.

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