Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase

G. C. Ness, C. E. Sample, M. Smith, L. C. Pendleton, D. C. Eichler

Research output: Contribution to journalArticle

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Abstract

1. A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. 2. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an M(r) of 100000 was observed. 3. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. 4. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.

Original languageEnglish (US)
Pages (from-to)167-172
Number of pages6
JournalBiochemical Journal
Volume233
Issue number1
DOIs
StatePublished - Jan 1 1986

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Hydroxymethylglutaryl CoA Reductases
Liver
Rats
Oxidoreductases
Glycosylation
Lysosomes
NADP
Immunoblotting
Immune Sera
Rabbits
Kinetics
3-hydroxy-3-methylglutaryl-coenzyme A

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ness, G. C. ; Sample, C. E. ; Smith, M. ; Pendleton, L. C. ; Eichler, D. C. / Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase. In: Biochemical Journal. 1986 ; Vol. 233, No. 1. pp. 167-172.
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Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase. / Ness, G. C.; Sample, C. E.; Smith, M.; Pendleton, L. C.; Eichler, D. C.

In: Biochemical Journal, Vol. 233, No. 1, 01.01.1986, p. 167-172.

Research output: Contribution to journalArticle

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