Characterization and mapping of the bovine FBP1 gene

H. Guo, Wansheng Liu, A. Takasuga, K. Eyer, E. Landrito, Shang zhong Xu, X. Gao, H. Y. Ren

Research output: Contribution to journalArticle

Abstract

Fructose-1,6-bisphosphatase (FBP1) is a key regulatory enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose-1,6-bisphosphate to generate fructose-6-phosphate and inorganic phosphate. Deficiency of fructose-1, 6-bisphosphatase is associated with fasting hypoglycemia and metabolic acidosis. The enzyme has been shown to occur in bacteria, fungi, plants and animals. The bovine FBP1 gene was cloned and characterized in this study. The full length (1,241 bp) FBP1 mRNA contained an open reading frame (ORF) encoding a protein of 338 amino acids, a 63 bp 5' untranslated region (UTR) and a 131 bp 3' UTR. The bovine FBP1 gene was 89%, 85%, 82%, 82% and 74% identical to the orthologs of pig, human, mouse, rat and zebra fish at mRNA level, and 97%, 96%, 94%, 93% and 91% identical at the protein level, respectively. This gene was broadly expressed in cattle with the highest level in testis, and the lowest level in heart. An intronic single nucleotide polymorphism (SNP) (A/G) was identified in the 5th intron of the bovine FBP1 gene. Genotyping of 133 animals from four beef breeds revealed that the average frequency for allele A (A-base) was 0.7897 (0.7069-0.9107), while 0.2103 (0.0893-0.2931) for allele B (G-base). Our preliminary association study indicated that this SNP is significantly associated with traits of Average Daily Feed Intake (ADFI) and Carcass Length (CL) (p<0.01). In addition, the FBP1 gene was assigned on BTA8 by a hybrid radiation (RH) mapping method.

Original languageEnglish (US)
Pages (from-to)1319-1326
Number of pages8
JournalAsian-Australasian Journal of Animal Sciences
Volume20
Issue number9
DOIs
StatePublished - Jan 1 2007

Fingerprint

cattle
fructose
Genes
genes
single nucleotide polymorphism
Fructose-1,6-Diphosphatase Deficiency
Single Nucleotide Polymorphism
Radiation Hybrid Mapping
Fructose-Bisphosphatase
fructose 6-phosphate
Messenger RNA
zebras
Gluconeogenesis
gluconeogenesis
hypoglycemia
5' Untranslated Regions
5' untranslated regions
3' Untranslated Regions
acidosis
Zebrafish

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology

Cite this

Guo, H., Liu, W., Takasuga, A., Eyer, K., Landrito, E., Xu, S. Z., ... Ren, H. Y. (2007). Characterization and mapping of the bovine FBP1 gene. Asian-Australasian Journal of Animal Sciences, 20(9), 1319-1326. https://doi.org/10.5713/ajas.2007.1319
Guo, H. ; Liu, Wansheng ; Takasuga, A. ; Eyer, K. ; Landrito, E. ; Xu, Shang zhong ; Gao, X. ; Ren, H. Y. / Characterization and mapping of the bovine FBP1 gene. In: Asian-Australasian Journal of Animal Sciences. 2007 ; Vol. 20, No. 9. pp. 1319-1326.
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Guo, H, Liu, W, Takasuga, A, Eyer, K, Landrito, E, Xu, SZ, Gao, X & Ren, HY 2007, 'Characterization and mapping of the bovine FBP1 gene', Asian-Australasian Journal of Animal Sciences, vol. 20, no. 9, pp. 1319-1326. https://doi.org/10.5713/ajas.2007.1319

Characterization and mapping of the bovine FBP1 gene. / Guo, H.; Liu, Wansheng; Takasuga, A.; Eyer, K.; Landrito, E.; Xu, Shang zhong; Gao, X.; Ren, H. Y.

In: Asian-Australasian Journal of Animal Sciences, Vol. 20, No. 9, 01.01.2007, p. 1319-1326.

Research output: Contribution to journalArticle

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AU - Liu, Wansheng

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AU - Xu, Shang zhong

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