TY - JOUR
T1 - Characterization and transposon mutagenesis of the maize (Zea mays) Pho1 Gene Family
AU - Salazar-Vidal, M. Nancy
AU - Acosta-Segovia, Edith
AU - Sanchez-León, Nidia
AU - Ahern, Kevin R.
AU - Brutnell, Thomas P.
AU - Sawers, Ruairidh J.H.
N1 - Funding Information:
We thank Juan M. Estévez-Palmas for valuable comments on the manuscript. This work was supported by the National Science Foundation grant IOS-0922701 to TB and the Mexican National Council of Science and Technology (CONACYT) grant CB2012-151947 to RS.
Publisher Copyright:
© 2016 Salazar-Vidal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/9
Y1 - 2016/9
N2 - Phosphorus is an essential nutrient for all plants, but also one of the least mobile, and consequently least available, in the soil. Plants have evolved a series of molecular, metabolic and developmental adaptations to increase the acquisition of phosphorus and to maximize the efficiency of use within the plant. In Arabidopsis (Arabidopsis thaliana), the AtPHO1 protein regulates and facilitates the distribution of phosphorus. To investigate the role of PHO1 proteins in maize (Zea mays), the B73 reference genome was searched for homologous sequences, and four genes identified that were designated ZmPho1; 1, ZmPho1; 2a, ZmPho1; 2b and ZmPho1; 3. ZmPho1; 2a and ZmPho1; 2b are the most similar to AtPHO1, and represent candidate co-orthologs that we hypothesize to have been retained following whole genome duplication. Evidence was obtained for the production of natural anti-sense transcripts associated with both ZmPho1; 2a and ZmPho1; 2b, suggesting the possibility of regulatory crosstalk between paralogs. To characterize functional divergence between ZmPho1; 2a and ZmPho1; 2b, a program of transposon mutagenesis was initiated using the AC/Ds system, and, here, we report the generation of novel alleles of ZmPho1; 2a and ZmPho1; 2b.
AB - Phosphorus is an essential nutrient for all plants, but also one of the least mobile, and consequently least available, in the soil. Plants have evolved a series of molecular, metabolic and developmental adaptations to increase the acquisition of phosphorus and to maximize the efficiency of use within the plant. In Arabidopsis (Arabidopsis thaliana), the AtPHO1 protein regulates and facilitates the distribution of phosphorus. To investigate the role of PHO1 proteins in maize (Zea mays), the B73 reference genome was searched for homologous sequences, and four genes identified that were designated ZmPho1; 1, ZmPho1; 2a, ZmPho1; 2b and ZmPho1; 3. ZmPho1; 2a and ZmPho1; 2b are the most similar to AtPHO1, and represent candidate co-orthologs that we hypothesize to have been retained following whole genome duplication. Evidence was obtained for the production of natural anti-sense transcripts associated with both ZmPho1; 2a and ZmPho1; 2b, suggesting the possibility of regulatory crosstalk between paralogs. To characterize functional divergence between ZmPho1; 2a and ZmPho1; 2b, a program of transposon mutagenesis was initiated using the AC/Ds system, and, here, we report the generation of novel alleles of ZmPho1; 2a and ZmPho1; 2b.
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U2 - 10.1371/journal.pone.0161882
DO - 10.1371/journal.pone.0161882
M3 - Article
C2 - 27648940
AN - SCOPUS:84992220428
SN - 1932-6203
VL - 11
JO - PLoS One
JF - PLoS One
IS - 9
M1 - e0161882
ER -