Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase β subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas

Vivek Kapur, Lingling Li, S. Iordanescu, M. R. Hamrick, A. Wanger, B. N. Kreiswirth, J. M. Musser

Research output: Contribution to journalComment/debate

267 Citations (Scopus)

Abstract

Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the β subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region and showed that most are missense mutations. The analysis also identified several mutant rpoB alleles not previously associated with resistant organisms and one short region of rpoB that had an unusually high frequency of insertions and deletions. Although many strains with an identical IS6110 restriction fragment length polymorphism pattern have the same variant rpoB allele, some do not, a result that suggests the occurrence of evolutionary divergence at the clone level.

Original languageEnglish (US)
Pages (from-to)1095-1098
Number of pages4
JournalJournal of clinical microbiology
Volume32
Issue number4
StatePublished - Jan 1 1994

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DNA-Directed RNA Polymerases
Rifampin
DNA Sequence Analysis
Mycobacterium tuberculosis
Alleles
Mutation
Missense Mutation
Restriction Fragment Length Polymorphisms
Genes
Clone Cells

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

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title = "Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase β subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas",
abstract = "Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the β subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90{\%} of rifampin-resistant strains have sequence alterations in this region and showed that most are missense mutations. The analysis also identified several mutant rpoB alleles not previously associated with resistant organisms and one short region of rpoB that had an unusually high frequency of insertions and deletions. Although many strains with an identical IS6110 restriction fragment length polymorphism pattern have the same variant rpoB allele, some do not, a result that suggests the occurrence of evolutionary divergence at the clone level.",
author = "Vivek Kapur and Lingling Li and S. Iordanescu and Hamrick, {M. R.} and A. Wanger and Kreiswirth, {B. N.} and Musser, {J. M.}",
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Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase β subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas. / Kapur, Vivek; Li, Lingling; Iordanescu, S.; Hamrick, M. R.; Wanger, A.; Kreiswirth, B. N.; Musser, J. M.

In: Journal of clinical microbiology, Vol. 32, No. 4, 01.01.1994, p. 1095-1098.

Research output: Contribution to journalComment/debate

TY - JOUR

T1 - Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase β subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas

AU - Kapur, Vivek

AU - Li, Lingling

AU - Iordanescu, S.

AU - Hamrick, M. R.

AU - Wanger, A.

AU - Kreiswirth, B. N.

AU - Musser, J. M.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the β subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region and showed that most are missense mutations. The analysis also identified several mutant rpoB alleles not previously associated with resistant organisms and one short region of rpoB that had an unusually high frequency of insertions and deletions. Although many strains with an identical IS6110 restriction fragment length polymorphism pattern have the same variant rpoB allele, some do not, a result that suggests the occurrence of evolutionary divergence at the clone level.

AB - Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the β subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region and showed that most are missense mutations. The analysis also identified several mutant rpoB alleles not previously associated with resistant organisms and one short region of rpoB that had an unusually high frequency of insertions and deletions. Although many strains with an identical IS6110 restriction fragment length polymorphism pattern have the same variant rpoB allele, some do not, a result that suggests the occurrence of evolutionary divergence at the clone level.

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