Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope

R. B.S. Roden, A. Armstrong, P. Haderer, Neil Christensen, N. L. Hubbert, D. R. Lowy, J. T. Schiller, R. Kirnbauer

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

We have determined that three type-specific and conformationally dependent monoclonal antibodies, H16.E70, H16.U4, and H16.V5, neutralize pseudotype human papillomavirus type 16 (HPV16) virions in vitro. H16.U4 and H16.V5 neutralized pseudotype virions derived from the German HPV16 variant 114K and the Zairian variant Z-1194 with equal efficiency. In contrast, neutralization of Z-1194 pseudotype virions by H16.E70 was two orders of magnitude weaker than neutralization of 114K pseudotype virions. This difference correlated with enzyme-linked immunosorbent assay reactivity of H16.E70 to L1 virus-like particles of the two variants. A substitution at residue 282 of L1 was responsible for this differential reactivity, suggesting that this residue constitutes part of the H16. E70 epitope.

Original languageEnglish (US)
Pages (from-to)6247-6252
Number of pages6
JournalJournal of Virology
Volume71
Issue number8
StatePublished - Aug 2 1997

Fingerprint

Human papillomavirus 16
Papillomaviridae
virion
neutralization
epitopes
Epitopes
Virion
virus-like particles
monoclonal antibodies
enzyme-linked immunosorbent assay
asoxime chloride
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Roden, R. B. S., Armstrong, A., Haderer, P., Christensen, N., Hubbert, N. L., Lowy, D. R., ... Kirnbauer, R. (1997). Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope. Journal of Virology, 71(8), 6247-6252.
Roden, R. B.S. ; Armstrong, A. ; Haderer, P. ; Christensen, Neil ; Hubbert, N. L. ; Lowy, D. R. ; Schiller, J. T. ; Kirnbauer, R. / Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope. In: Journal of Virology. 1997 ; Vol. 71, No. 8. pp. 6247-6252.
@article{d75d6e080b4844e6a9eb821219a552a9,
title = "Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope",
abstract = "We have determined that three type-specific and conformationally dependent monoclonal antibodies, H16.E70, H16.U4, and H16.V5, neutralize pseudotype human papillomavirus type 16 (HPV16) virions in vitro. H16.U4 and H16.V5 neutralized pseudotype virions derived from the German HPV16 variant 114K and the Zairian variant Z-1194 with equal efficiency. In contrast, neutralization of Z-1194 pseudotype virions by H16.E70 was two orders of magnitude weaker than neutralization of 114K pseudotype virions. This difference correlated with enzyme-linked immunosorbent assay reactivity of H16.E70 to L1 virus-like particles of the two variants. A substitution at residue 282 of L1 was responsible for this differential reactivity, suggesting that this residue constitutes part of the H16. E70 epitope.",
author = "Roden, {R. B.S.} and A. Armstrong and P. Haderer and Neil Christensen and Hubbert, {N. L.} and Lowy, {D. R.} and Schiller, {J. T.} and R. Kirnbauer",
year = "1997",
month = "8",
day = "2",
language = "English (US)",
volume = "71",
pages = "6247--6252",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "8",

}

Roden, RBS, Armstrong, A, Haderer, P, Christensen, N, Hubbert, NL, Lowy, DR, Schiller, JT & Kirnbauer, R 1997, 'Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope', Journal of Virology, vol. 71, no. 8, pp. 6247-6252.

Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope. / Roden, R. B.S.; Armstrong, A.; Haderer, P.; Christensen, Neil; Hubbert, N. L.; Lowy, D. R.; Schiller, J. T.; Kirnbauer, R.

In: Journal of Virology, Vol. 71, No. 8, 02.08.1997, p. 6247-6252.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope

AU - Roden, R. B.S.

AU - Armstrong, A.

AU - Haderer, P.

AU - Christensen, Neil

AU - Hubbert, N. L.

AU - Lowy, D. R.

AU - Schiller, J. T.

AU - Kirnbauer, R.

PY - 1997/8/2

Y1 - 1997/8/2

N2 - We have determined that three type-specific and conformationally dependent monoclonal antibodies, H16.E70, H16.U4, and H16.V5, neutralize pseudotype human papillomavirus type 16 (HPV16) virions in vitro. H16.U4 and H16.V5 neutralized pseudotype virions derived from the German HPV16 variant 114K and the Zairian variant Z-1194 with equal efficiency. In contrast, neutralization of Z-1194 pseudotype virions by H16.E70 was two orders of magnitude weaker than neutralization of 114K pseudotype virions. This difference correlated with enzyme-linked immunosorbent assay reactivity of H16.E70 to L1 virus-like particles of the two variants. A substitution at residue 282 of L1 was responsible for this differential reactivity, suggesting that this residue constitutes part of the H16. E70 epitope.

AB - We have determined that three type-specific and conformationally dependent monoclonal antibodies, H16.E70, H16.U4, and H16.V5, neutralize pseudotype human papillomavirus type 16 (HPV16) virions in vitro. H16.U4 and H16.V5 neutralized pseudotype virions derived from the German HPV16 variant 114K and the Zairian variant Z-1194 with equal efficiency. In contrast, neutralization of Z-1194 pseudotype virions by H16.E70 was two orders of magnitude weaker than neutralization of 114K pseudotype virions. This difference correlated with enzyme-linked immunosorbent assay reactivity of H16.E70 to L1 virus-like particles of the two variants. A substitution at residue 282 of L1 was responsible for this differential reactivity, suggesting that this residue constitutes part of the H16. E70 epitope.

UR - http://www.scopus.com/inward/record.url?scp=0030745301&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030745301&partnerID=8YFLogxK

M3 - Article

VL - 71

SP - 6247

EP - 6252

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 8

ER -

Roden RBS, Armstrong A, Haderer P, Christensen N, Hubbert NL, Lowy DR et al. Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope. Journal of Virology. 1997 Aug 2;71(8):6247-6252.