Abstract
From a pollen tube cDNA library of Petunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing the PPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDNA and genomic sequences revealed the presence of a single intron in the PPE1 gene. During pollen development, PPE1 mRNA was first detected in anthers containing uninucleate microspores; it reached the highest level in mature pollen and persisted at a high level in in vitro germinated pollen tubes. The observed expression pattern of the PPE1 gene suggests that its product may play a role in pollen germination and/or tube growth.
Original language | English (US) |
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Pages (from-to) | 539-544 |
Number of pages | 6 |
Journal | Plant molecular biology |
Volume | 25 |
Issue number | 3 |
DOIs | |
State | Published - Jun 1994 |
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Genetics
- Plant Science