Characterization of a pollen-expressed gene encoding a putative pectin esterase of Petunia inflata

Jing Hong Mu, Joseph P. Stains, Teh-hui Kao

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Abstract

From a pollen tube cDNA library of Petunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing the PPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDNA and genomic sequences revealed the presence of a single intron in the PPE1 gene. During pollen development, PPE1 mRNA was first detected in anthers containing uninucleate microspores; it reached the highest level in mature pollen and persisted at a high level in in vitro germinated pollen tubes. The observed expression pattern of the PPE1 gene suggests that its product may play a role in pollen germination and/or tube growth.

Original languageEnglish (US)
Pages (from-to)539-544
Number of pages6
JournalPlant molecular biology
Volume25
Issue number3
DOIs
StatePublished - Jun 1 1994

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

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