Characterization of a pollen-expressed receptor-like kinase gene of Petunia inflata and the activity of its encoded kinase

Jing Hong Mu, Hyun Sook Lee, Teh Hui Kao

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

From a pollen tube cDNA library of Petunia inflata, we isolated clones encoding a protein with structural features and biochemical properties characteristic of receptor-like kinases. It was designated PRK1 for pollen receptor-like kinase 1. The cytoplasmic domain of PRK1 is highly similar to the kinase domains of other plant receptor-like kinases and contains nearly all of the conserved amino acids for serine/threonine kinases. The extracellular domain of PRK1 contains leucine-rich repeats as found in some other plant receptor-like kinases, but overall its sequence in this region does not share significant similarity. Characterization of a gene encoding PRK1 revealed the presence of two introns. During pollen development, PRK1 mRNA was first detected in anthers containing mostly binucleate microspores; it reached the highest level of mature pollen and remained at a high level in in vitro-germinated pollen tubes. The recombinant cytoplasmic domain of PRK1 autophosphorylated on serine and tyrosine, suggesting that PRK1 may be a dual-specificity kinase. Monospecific immune serum to the recombinant extracellular domain of PRK1 detected a 69-kD protein in microsomal membranes of pollen and pollen tubes. The characteristics of PRK1 suggest that it may play a role in signal transduction events during pollen development and/or pollination.

Original languageEnglish (US)
Pages (from-to)709-721
Number of pages13
JournalPlant Cell
Volume6
Issue number5
StatePublished - May 1994

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

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