Characterization of a salivary lysozyme in larval Helicoverpa zea

Feng Liu, Liwang Cui, Diana Lynn Cox-foster, Gary Felton

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The cDNA sequence of a salivary lysozyme in Helicoverpa zea (Lepidoptera: Noctuidae) was determined. The full-length cDNA is 1,032 bp, and it encodes a protein of 142 amino acids. This lysozyme has 90% identity with Heliothis virescens lysozyme and 76% identity with Manduca sexta lysozyme. There is a signal peptide of 20 amino acids at the N-terminus. The mature protein is about 14.4 kDa without the signal peptide. The pI value is greater than 9.5 as determined by isoelectric focusing. From genomic DNA, two introns and three exons were within the open reading frame (ORF). Southern blot analysis indicated that it is a single-copy gene. A time-course study revealed that the H. zea lysozyme gene was differentially expressed in the labial glands during the development of fifth-instar larvae, with the peak level of lysozyme mRNA being detected on day 1. Dot blot analysis showed different levels of H. zea lysozyme expression when the caterpillars fed on different plants. Further, the H. zea lysozyme could be detected with antibodies raised against the M. sexta lysozyme, and it was one of the most abundant secreted proteins in saliva collected directly from the caterpillar's spinneret. The potential role of the lysozyme on host plants in mediating susceptibility to bacterial disease is discussed in the context of tritrophic interactions.

Original languageEnglish (US)
Pages (from-to)2439-2457
Number of pages19
JournalJournal of Chemical Ecology
Volume30
Issue number12
DOIs
StatePublished - Dec 1 2004

Fingerprint

Helicoverpa zea
Muramidase
lysozyme
Zea mays
caterpillar
peptide
protein
amino acid
tritrophic interaction
bacterial disease
saliva
gene
antibody
host plant
genomics
Manduca
larva
Manduca sexta
DNA
Protein Sorting Signals

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

Liu, Feng ; Cui, Liwang ; Cox-foster, Diana Lynn ; Felton, Gary. / Characterization of a salivary lysozyme in larval Helicoverpa zea. In: Journal of Chemical Ecology. 2004 ; Vol. 30, No. 12. pp. 2439-2457.
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Characterization of a salivary lysozyme in larval Helicoverpa zea. / Liu, Feng; Cui, Liwang; Cox-foster, Diana Lynn; Felton, Gary.

In: Journal of Chemical Ecology, Vol. 30, No. 12, 01.12.2004, p. 2439-2457.

Research output: Contribution to journalArticle

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