Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features

Chisato Shimizu, Hiroko Shike, Denise M. Malicki, Eric Breisch, Mark Westerman, John Buchanan, Heather R. Ligman, Ruth B. Phillips, James M. Carlberg, Jack Van Olst, Jane C. Burns

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Abstract

The genus Morone is an important one for U.S. aquaculture, but there has been no available cell line from this genus. We report here a cell line (the WBE line) derived from white bass embryos that has been grown for more than 80 passages over 21 mo in Dulbecco modified Eagle medium supplemented with fetal bovine serum. The WBE line showed epithelial features with positive immunohistochemical staining for cytokeratin and intercellular junctions when observed by electron microscopy. The doubling time, transformation characteristics, response to cryopreservation, and karyotype were determined. The WBE line was also tested by polymerase chain reaction to verify the species of origin and to screen for mycoplasma infection. This epithelial cell line may prove useful for the study of host-pathogen interactions that occur at the epithelial surface in this commercially important fish species.

Original languageEnglish (US)
Pages (from-to)29-35
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume39
Issue number1-2
DOIs
StatePublished - Jan 1 2003

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All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Shimizu, C., Shike, H., Malicki, D. M., Breisch, E., Westerman, M., Buchanan, J., Ligman, H. R., Phillips, R. B., Carlberg, J. M., Van Olst, J., & Burns, J. C. (2003). Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cellular and Developmental Biology - Animal, 39(1-2), 29-35. https://doi.org/10.1290/1543-706X(2003)039<0029:COAWBM>2.0.CO;2