Characterization of an HPV type 11 isolate propagated in human foreskin implants in nude mice

S. C. Dollard, L. T. Chow, J. W. Kreider, T. R. Broker, Nancy Lill, M. K. Howett

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Human papillomavirus type 11-Hershey (HPV-11-H) from an extract of genital warts has been serially passaged by infecting human foreskin chips that are then implanted in athymic mice (1. W. Kreider, M. K. Howett, A. E. Leure-Dupree, R. J. Zaino, and J. A. Weber (1987), J. Virol. 61, 590). Subsequent attempts to propagate HPVs present in other human lesions have not been successful. In an effort to identify the basis for the seemingly unique ability of HPV-11-H to propagate in the xenografts, we carried out extensive physical and functional characterizations of the cloned DNA, including restriction digestions, DNA sequencing of transcriptional control regions, and E2 trans-activation of the upstream regulatory region. We uncovered no significant differences compared to the prototype HPV-11 (K. Dartmann, E. Schwarz, L. Gissmann, and H. zur Hausen (1986) Virology 151,124; H. Hirochika, T. R. Broker, and L. T. Chow (1987) J. Virol. 61, 2599). Moreover, viral enhancer assays indicated that the observed stimulatory effect of pregnancy on condylomata and of estradiol on experimental cysts is likely an indirect one and could not be attributed to up-regulation of the HPV-11 transcriptional enhancer.

Original languageEnglish (US)
Pages (from-to)294-297
Number of pages4
JournalVirology
Volume171
Issue number1
DOIs
StatePublished - Jan 1 1989

Fingerprint

Human papillomavirus 11
Foreskin
Nude Mice
Condylomata Acuminata
Virology
Nucleic Acid Regulatory Sequences
DNA Sequence Analysis
Heterografts
Cysts
Digestion
Estradiol
Up-Regulation
Pregnancy
DNA

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Dollard, S. C., Chow, L. T., Kreider, J. W., Broker, T. R., Lill, N., & Howett, M. K. (1989). Characterization of an HPV type 11 isolate propagated in human foreskin implants in nude mice. Virology, 171(1), 294-297. https://doi.org/10.1016/0042-6822(89)90542-4
Dollard, S. C. ; Chow, L. T. ; Kreider, J. W. ; Broker, T. R. ; Lill, Nancy ; Howett, M. K. / Characterization of an HPV type 11 isolate propagated in human foreskin implants in nude mice. In: Virology. 1989 ; Vol. 171, No. 1. pp. 294-297.
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Characterization of an HPV type 11 isolate propagated in human foreskin implants in nude mice. / Dollard, S. C.; Chow, L. T.; Kreider, J. W.; Broker, T. R.; Lill, Nancy; Howett, M. K.

In: Virology, Vol. 171, No. 1, 01.01.1989, p. 294-297.

Research output: Contribution to journalArticle

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