Characterization of an iron-sulfur flavoprotein from Methanosarcina thermophila

Matthew T. Latimer, Michael H. Painter, James Gregory Ferry

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A gene (isf) encoding an iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila was cloned and sequenced. The gene was located directly upstream of the genes (pta and ack) encoding phosphotransacetylase and acetate kinase and is transcribed in the opposite direction. The amino acid sequence deduced from isf contained a cluster of cysteine residues reminiscent of proteins that accommodate either a [4Fe-4S] or [3Fe-4S] center. The protein was heterologously produced in Escherichia coli and purified to apparent homogeneity. The 29-kDa subunit molecular mass of heterologously produced Isf (determined by SDS-polyacrylamide gel electrophoresis) corresponded to the molecular mass of 30,451 Da calculated from the amino acid composition deduced from isf. Gel filtration estimated a molecular mass of 65 kDa for the native Isf indicating an α2 homodimer. The UV-visible absorption spectrum was characteristic of iron-sulfur flavoproteins with maxima at 484, 452, 430, 378, and 280 nm. Analyses identified 2 FMN, 7-8 non-heme iron atoms, and 6-7 acid-labile sulfur atoms per α2 homodimer. Comparisons of the deduced Isf sequence with sequences in available protein data bases suggested Isf is a novel iron-sulfur flavoprotein. Western blot analysis indicated the presence of Isf in extracts of acetate-grown M. thermophila. Ferredoxin stimulated the CO-dependant reduction of Isf by the CO dehydrogenase-acetyl-CoA synthase complex that suggested ferredoxin is a physiological electron donor to Isf.

Original languageEnglish (US)
Pages (from-to)24023-24028
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number39
DOIs
StatePublished - Oct 10 1996

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Flavoproteins
Sulfur
Iron
Molecular mass
Ferredoxins
carbon monoxide dehydrogenase
Phosphate Acetyltransferase
Sulfur Acids
Acetate Kinase
Genes
Methanosarcina thermophila Isf protein
Flavin Mononucleotide
Amino Acids
Atoms
Acetyl Coenzyme A
Proteins
Gene encoding
Carbon Monoxide
Electrophoresis
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Latimer, Matthew T. ; Painter, Michael H. ; Ferry, James Gregory. / Characterization of an iron-sulfur flavoprotein from Methanosarcina thermophila. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 39. pp. 24023-24028.
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abstract = "A gene (isf) encoding an iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila was cloned and sequenced. The gene was located directly upstream of the genes (pta and ack) encoding phosphotransacetylase and acetate kinase and is transcribed in the opposite direction. The amino acid sequence deduced from isf contained a cluster of cysteine residues reminiscent of proteins that accommodate either a [4Fe-4S] or [3Fe-4S] center. The protein was heterologously produced in Escherichia coli and purified to apparent homogeneity. The 29-kDa subunit molecular mass of heterologously produced Isf (determined by SDS-polyacrylamide gel electrophoresis) corresponded to the molecular mass of 30,451 Da calculated from the amino acid composition deduced from isf. Gel filtration estimated a molecular mass of 65 kDa for the native Isf indicating an α2 homodimer. The UV-visible absorption spectrum was characteristic of iron-sulfur flavoproteins with maxima at 484, 452, 430, 378, and 280 nm. Analyses identified 2 FMN, 7-8 non-heme iron atoms, and 6-7 acid-labile sulfur atoms per α2 homodimer. Comparisons of the deduced Isf sequence with sequences in available protein data bases suggested Isf is a novel iron-sulfur flavoprotein. Western blot analysis indicated the presence of Isf in extracts of acetate-grown M. thermophila. Ferredoxin stimulated the CO-dependant reduction of Isf by the CO dehydrogenase-acetyl-CoA synthase complex that suggested ferredoxin is a physiological electron donor to Isf.",
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Characterization of an iron-sulfur flavoprotein from Methanosarcina thermophila. / Latimer, Matthew T.; Painter, Michael H.; Ferry, James Gregory.

In: Journal of Biological Chemistry, Vol. 271, No. 39, 10.10.1996, p. 24023-24028.

Research output: Contribution to journalArticle

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