Characterization of an S-locus receptor protein kinase-like gene from peach

Carole L. Bassett, Michael L. Nickerson, Robert E. Farrell, Jr., Timothy S. Artlip, Ahmed El Ghaouth, Charles L. Wilson, Michael E. Wisniewski

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A receptor-like protein kinase gene (Ppsrkl1) was isolated from a peach (Prunus persica (L.) Batsch.) bark cDNA library prepared with RNAs isolated from bark collected in December (cold acclimated). Sequence analysis indicated that this gene is related to the S-locus family of receptor protein kinases (SRKs) and that it shares greatest homology with ZMPK1 from maize and At4g32300 from Arabidopsis, both of which are intron-less genes. In bark tissues, Ppsrkl1 is induced by water deficit treatment, repressed by short-day photoperiods and showed no response to cold treatment. The Ppsrkl1 mRNA also increased in roots in response to water deficit. In fruit, Ppsrkl1 shows no response up to 6 h after wounding, but at 12 and 24 h after wounding, Ppsrkl1 mRNA shows an abrupt decline. This decline was prevented by the addition of salicylic acid to the wound site. The Ppsrkl1 mRNA rapidly decreased in fruit after 10-min exposure to UV-C radiation, followed by a return to normal levels within 1.5 h. Taken together, these experiments indicate that Ppsrkl1 is negatively regulated by light and positively influenced by salicylic acid treatment in fruit and water stress in bark and roots.

Original languageEnglish (US)
Pages (from-to)403-411
Number of pages9
JournalTree Physiology
Volume25
Issue number4
DOIs
StatePublished - Jan 1 2005

Fingerprint

protein kinases
Protein Kinases
peaches
Fruit
bark
Salicylic Acid
Messenger RNA
receptors
loci
salicylic acid
Genes
fruits
genes
Water Purification
Photoperiod
Gene Library
Dehydration
Arabidopsis
Introns
Zea mays

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

Bassett, C. L., Nickerson, M. L., Farrell, Jr., R. E., Artlip, T. S., El Ghaouth, A., Wilson, C. L., & Wisniewski, M. E. (2005). Characterization of an S-locus receptor protein kinase-like gene from peach. Tree Physiology, 25(4), 403-411. https://doi.org/10.1093/treephys/25.4.403
Bassett, Carole L. ; Nickerson, Michael L. ; Farrell, Jr., Robert E. ; Artlip, Timothy S. ; El Ghaouth, Ahmed ; Wilson, Charles L. ; Wisniewski, Michael E. / Characterization of an S-locus receptor protein kinase-like gene from peach. In: Tree Physiology. 2005 ; Vol. 25, No. 4. pp. 403-411.
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abstract = "A receptor-like protein kinase gene (Ppsrkl1) was isolated from a peach (Prunus persica (L.) Batsch.) bark cDNA library prepared with RNAs isolated from bark collected in December (cold acclimated). Sequence analysis indicated that this gene is related to the S-locus family of receptor protein kinases (SRKs) and that it shares greatest homology with ZMPK1 from maize and At4g32300 from Arabidopsis, both of which are intron-less genes. In bark tissues, Ppsrkl1 is induced by water deficit treatment, repressed by short-day photoperiods and showed no response to cold treatment. The Ppsrkl1 mRNA also increased in roots in response to water deficit. In fruit, Ppsrkl1 shows no response up to 6 h after wounding, but at 12 and 24 h after wounding, Ppsrkl1 mRNA shows an abrupt decline. This decline was prevented by the addition of salicylic acid to the wound site. The Ppsrkl1 mRNA rapidly decreased in fruit after 10-min exposure to UV-C radiation, followed by a return to normal levels within 1.5 h. Taken together, these experiments indicate that Ppsrkl1 is negatively regulated by light and positively influenced by salicylic acid treatment in fruit and water stress in bark and roots.",
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Bassett, CL, Nickerson, ML, Farrell, Jr., RE, Artlip, TS, El Ghaouth, A, Wilson, CL & Wisniewski, ME 2005, 'Characterization of an S-locus receptor protein kinase-like gene from peach', Tree Physiology, vol. 25, no. 4, pp. 403-411. https://doi.org/10.1093/treephys/25.4.403

Characterization of an S-locus receptor protein kinase-like gene from peach. / Bassett, Carole L.; Nickerson, Michael L.; Farrell, Jr., Robert E.; Artlip, Timothy S.; El Ghaouth, Ahmed; Wilson, Charles L.; Wisniewski, Michael E.

In: Tree Physiology, Vol. 25, No. 4, 01.01.2005, p. 403-411.

Research output: Contribution to journalArticle

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