Characterization of Bacterial Phospholipids by Electrospray Ionization Tandem Mass Spectrometry

Philip B. Smith, A. Peter Snyder, Charles S. Harden

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

A negative ion electrospray ionization tandem mass spectrometric technique was developed for the analysis of glycerophospholipids. Examination of the product ion mass spectrum of the deprotonated molecular ion provided sufficient information to identify both the class of glycerophospholipid and the molecular weights of the two fatty acid moieties. This technique was applied to the profiling of glycerophospholipids present in the chloroform/methanol extracts of four different bacterial species. The principal bacterial phospholipids detected by this technique were phosphatidylglycerols and diphosphatidylglycerols, accompanied by small amounts of phosphatidylethanolamines for two of the bacterial species examined. The fatty acid composition of the phosphatidylglycerols for each bacteria was determined by tandem mass spectrometry and presented graphically. Differences in the fatty acid composition for each bacterial species were readily apparent from a visual examination of the data sets.

Original languageEnglish (US)
Pages (from-to)1824-1830
Number of pages7
JournalAnalytical chemistry
Volume67
Issue number11
DOIs
StatePublished - Jan 1 1995

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Glycerophospholipids
Electrospray ionization
Mass spectrometry
Phospholipids
Phosphatidylglycerols
Fatty Acids
Phosphatidylethanolamines
Ions
Cardiolipins
Chloroform
Chemical analysis
Methanol
Bacteria
Negative ions
Molecular weight

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Smith, Philip B. ; Snyder, A. Peter ; Harden, Charles S. / Characterization of Bacterial Phospholipids by Electrospray Ionization Tandem Mass Spectrometry. In: Analytical chemistry. 1995 ; Vol. 67, No. 11. pp. 1824-1830.
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Characterization of Bacterial Phospholipids by Electrospray Ionization Tandem Mass Spectrometry. / Smith, Philip B.; Snyder, A. Peter; Harden, Charles S.

In: Analytical chemistry, Vol. 67, No. 11, 01.01.1995, p. 1824-1830.

Research output: Contribution to journalArticle

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