TY - JOUR
T1 - Characterization of Ca-phosphate biological materials by scanning transmission X-ray microscopy (STXM) at the Ca L2,3-, P L2,3- And C K-edges
AU - Cosmidis, Julie
AU - Benzerara, Karim
AU - Nassif, Nadine
AU - Tyliszczak, Tolek
AU - Bourdelle, Franck
N1 - Funding Information:
We thank Florence Babonneau, Yan Wang, Stanislas Von Euw, Christian Bonhomme and Frédérique Pourpoint (Collège de France and UPMC, Paris), for providing some of the reference Ca-phosphates used in this study. Haohao Yi and Etienne Balan (IMPMC, Paris) are also thanked for providing compounds and analyses. We gratefully acknowledge support from the Simone and Cino Del Duca Fundation for funding J.C.’s salary. Part of the research leading to these results received funding from the European Research Council under the European Community’s Seventh Framework Programme (FP7/2007-2013 Grant Agreement no. 307110 – ERC CALCYAN ). Advanced Light Source (ALS) Molecular Environmental Science beamline 11.0.2 is supported by the Office of Science, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences and Materials Sciences Division, U.S. Department of Energy , at the Lawrence Berkeley National Laboratory. Beamline 10ID-1 at the CLS is supported by the NSERC , the CIHR , the NRC and the University of Saskatchewan . We thank Chithra Karunakaran and Jian Wang for their support of the STXM at the CLS. The authors are indebted to Hannah Miller (University of Colorado) for proofreading this article.
Publisher Copyright:
© 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Several naturally occurring biological materials, including bones and teeth, pathological calcifications, microbial mineral deposits formed in marine phosphogenesis areas, as well as bio-inspired cements used for bone and tooth repair are composed of Ca-phosphates. These materials are usually identified and characterized using bulk-scale analytical tools such as X-ray diffraction, Fourier transform infrared spectroscopy or nuclear magnetic resonance. However, there is a need for imaging techniques that provide information on the spatial distribution and chemical composition of the Ca-phosphate phases at the micrometer- and nanometer scales. Such analyses provide insightful indications on how the materials may have formed, e.g. through transient precursor phases that eventually remain spatially separated from the mature phase. Here, we present scanning transmission X-ray microscopy (STXM) analyses of Ca-phosphate reference compounds, showing the feasibility of fingerprinting Ca-phosphate-based materials. We calibrate methods to determine important parameters of Ca-phosphate phases, such as their Ca/P ratio and carbonate content at the ∼25 nm scale, using X-ray absorption near-edge spectra at the C K-, Ca L2,3- and P L2,3-edges. As an illustrative case study, we also perform STXM analyses on hydroxyapatite precipitates formed in a dense fibrillar collagen matrix. This study paves the way for future research on Ca-phosphate biomineralization processes down to the scale of a few tens of nanometers.
AB - Several naturally occurring biological materials, including bones and teeth, pathological calcifications, microbial mineral deposits formed in marine phosphogenesis areas, as well as bio-inspired cements used for bone and tooth repair are composed of Ca-phosphates. These materials are usually identified and characterized using bulk-scale analytical tools such as X-ray diffraction, Fourier transform infrared spectroscopy or nuclear magnetic resonance. However, there is a need for imaging techniques that provide information on the spatial distribution and chemical composition of the Ca-phosphate phases at the micrometer- and nanometer scales. Such analyses provide insightful indications on how the materials may have formed, e.g. through transient precursor phases that eventually remain spatially separated from the mature phase. Here, we present scanning transmission X-ray microscopy (STXM) analyses of Ca-phosphate reference compounds, showing the feasibility of fingerprinting Ca-phosphate-based materials. We calibrate methods to determine important parameters of Ca-phosphate phases, such as their Ca/P ratio and carbonate content at the ∼25 nm scale, using X-ray absorption near-edge spectra at the C K-, Ca L2,3- and P L2,3-edges. As an illustrative case study, we also perform STXM analyses on hydroxyapatite precipitates formed in a dense fibrillar collagen matrix. This study paves the way for future research on Ca-phosphate biomineralization processes down to the scale of a few tens of nanometers.
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U2 - 10.1016/j.actbio.2014.10.003
DO - 10.1016/j.actbio.2014.10.003
M3 - Article
C2 - 25305511
AN - SCOPUS:84925064807
SN - 1742-7061
VL - 12
SP - 260
EP - 269
JO - Acta Biomaterialia
JF - Acta Biomaterialia
IS - 1
ER -