Characterization of CKβ8 and CKβ8-1

Two alternatively spliced forms of human β-chemokine, chemoattractants for neutrophils, monocytes, and lymphocytes, and potent agonists at CC chemokine receptor 1

Byung S. Youn, Shangming Zhang, Hal E. Broxmeyer, Scott Cooper, Kathleen Antol, Malcolm Fraser, Byoung S. Kwon

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Two new members of human β-chemokine cDNA were isolated based on structural and functional similarities to human leukotactin-1. One of these clones was identical to the previously isolated human β-chemokine, CKβ8, whereas the other is a splicing variant of CKβ8, therefore named CKβ8-1. CKβ8 was short in 51 nucleotides (17 amino acids) compared with CKβ8-1. The mature proteins of CKβ8-1 and CKβ8 consisted of 116 and 99 amino acids with calculated molecular weights of 12,500 and 10,950, respectively. Both CKβ8- 1 and CKβ8 were potent agonists at CCR1. These chemokines chemoattracted neutrophils, monocytes, and lymphocytes. They also significantly suppressed colony formation by human bone marrow, granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. To our knowledge, this is the first example that an alternative splicing produces two active β-chemokines from a single gene.

Original languageEnglish (US)
Pages (from-to)3118-3126
Number of pages9
JournalBlood
Volume91
Issue number9
StatePublished - May 1 1998

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CCR1 Receptors
Lymphocytes
Chemotactic Factors
Chemokines
Monocytes
Neutrophils
Amino Acids
Erythroid Precursor Cells
Macrophages
Alternative Splicing
Granulocytes
Intercellular Signaling Peptides and Proteins
Bone
Nucleotides
Complementary DNA
Clone Cells
Genes
Molecular Weight
Bone Marrow
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Youn, Byung S. ; Zhang, Shangming ; Broxmeyer, Hal E. ; Cooper, Scott ; Antol, Kathleen ; Fraser, Malcolm ; Kwon, Byoung S. / Characterization of CKβ8 and CKβ8-1 : Two alternatively spliced forms of human β-chemokine, chemoattractants for neutrophils, monocytes, and lymphocytes, and potent agonists at CC chemokine receptor 1. In: Blood. 1998 ; Vol. 91, No. 9. pp. 3118-3126.
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Characterization of CKβ8 and CKβ8-1 : Two alternatively spliced forms of human β-chemokine, chemoattractants for neutrophils, monocytes, and lymphocytes, and potent agonists at CC chemokine receptor 1. / Youn, Byung S.; Zhang, Shangming; Broxmeyer, Hal E.; Cooper, Scott; Antol, Kathleen; Fraser, Malcolm; Kwon, Byoung S.

In: Blood, Vol. 91, No. 9, 01.05.1998, p. 3118-3126.

Research output: Contribution to journalArticle

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