Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus

M. V. Graves, Marilyn J. Roossinck

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Two defective RNAs (designated D RNA 3α and D RNA 3β) were found to be associated with the Fny strain of cucumber mosaic cucumovirus but not with the Sny strain after serial passages in a tobacco host. The D RNAs were derived from RNA 3 by single, in-frame deletions within the 3a open reading frame. A full-length cDNA clone from which biologically active transcripts can be produced in vitro has been constructed for D RNA 3β. This transcript can be replicated in tobacco plants infected with subgroup I and II cucumber mosaic cucumovirus strains and with peanut stunt cucumovirus. Translation of D RNA 3β in vitro produced a 20-kDa peptide, which was consistent with the predicted coding capacity of the deleted 3a open reading frame. D RNA 3β was also associated with polyribosomes isolated from infected tobacco plants. The presence of the D RNAs had no apparent effect upon helper virus yield or symptom production.

Original languageEnglish (US)
Pages (from-to)4746-4751
Number of pages6
JournalJournal of Virology
Volume69
Issue number8
StatePublished - 1995

Fingerprint

Cucumovirus
Cucumis sativus
Cucumber mosaic virus
RNA
Tobacco
tobacco
Open Reading Frames
Helper Viruses
open reading frames
Serial Passage
Peanut stunt virus
Polyribosomes
DNA-like RNA
polyribosomes
Complementary DNA
Clone Cells
signs and symptoms (plants)
translation (genetics)
Peptides
peptides

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

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Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus. / Graves, M. V.; Roossinck, Marilyn J.

In: Journal of Virology, Vol. 69, No. 8, 1995, p. 4746-4751.

Research output: Contribution to journalArticle

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