Characterization of egg laying hen and broiler fecal microbiota in poultry farms in Croatia, Czech Republic, Hungary and Slovenia

Petra Videnska, Md Masudur Rahman, Marcela Faldynova, Vladimir Babak, Marta Elsheimer Matulova, Estella Prukner-Radovcic, Ivan Krizek, Sonja Smole-Mozina, Jasna Kovac, Ama Szmolka, Bela Nagy, Karel Sedlar, Darina Cejkova, Ivan Rychlik

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Poultry meat is the most common protein source of animal origin for humans. However, intensive breeding of animals in confined spaces has led to poultry colonisation by microbiota with a zoonotic potential or encoding antibiotic resistances. In this study we were therefore interested in the prevalence of selected antibiotic resistance genes and microbiota composition in feces of egg laying hens and broilers originating from 4 different Central European countries determined by real-time PCR and 16S rRNA gene pyrosequencing, respectively. strA gene was present in 1 out of 10,000 bacteria. The prevalence of sul1, sul2 and tet(B) in poultry microbiota was approx. 6 times lower than that of the strA gene. tet(A) and cat were the least prevalent being present in around 3 out of 10,000,000 bacteria forming fecal microbiome. The core chicken fecal microbiota was formed by 26 different families. Rather unexpectedly, representatives of Desulfovibrionaceae and Campylobacteraceae , both capable of hydrogen utilisation in complex microbial communities, belonged among core microbiota families. Understanding the roles of individual population members in the total metabolism of the complex community may allow for interventions which might result in the replacement of Campylobacteraceae with Desulfovibrionaceae and a reduction of Campylobacter colonisation in broilers, carcasses, and consequently poultry meat products.

Original languageEnglish (US)
Article numbere110076
JournalPloS one
Volume9
Issue number10
DOIs
StatePublished - Oct 16 2014

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Slovenia
Poultry
Croatia
Hungary
Czech Republic
Microbiota
laying hens
Farms
Ovum
poultry
oviposition
Genes
broiler chickens
farms
Desulfovibrionaceae
Campylobacteraceae
Meats
Bacteria
Animals
poultry meat

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Videnska, P., Rahman, M. M., Faldynova, M., Babak, V., Matulova, M. E., Prukner-Radovcic, E., ... Rychlik, I. (2014). Characterization of egg laying hen and broiler fecal microbiota in poultry farms in Croatia, Czech Republic, Hungary and Slovenia. PloS one, 9(10), [e110076]. https://doi.org/10.1371/journal.pone.0110076
Videnska, Petra ; Rahman, Md Masudur ; Faldynova, Marcela ; Babak, Vladimir ; Matulova, Marta Elsheimer ; Prukner-Radovcic, Estella ; Krizek, Ivan ; Smole-Mozina, Sonja ; Kovac, Jasna ; Szmolka, Ama ; Nagy, Bela ; Sedlar, Karel ; Cejkova, Darina ; Rychlik, Ivan. / Characterization of egg laying hen and broiler fecal microbiota in poultry farms in Croatia, Czech Republic, Hungary and Slovenia. In: PloS one. 2014 ; Vol. 9, No. 10.
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abstract = "Poultry meat is the most common protein source of animal origin for humans. However, intensive breeding of animals in confined spaces has led to poultry colonisation by microbiota with a zoonotic potential or encoding antibiotic resistances. In this study we were therefore interested in the prevalence of selected antibiotic resistance genes and microbiota composition in feces of egg laying hens and broilers originating from 4 different Central European countries determined by real-time PCR and 16S rRNA gene pyrosequencing, respectively. strA gene was present in 1 out of 10,000 bacteria. The prevalence of sul1, sul2 and tet(B) in poultry microbiota was approx. 6 times lower than that of the strA gene. tet(A) and cat were the least prevalent being present in around 3 out of 10,000,000 bacteria forming fecal microbiome. The core chicken fecal microbiota was formed by 26 different families. Rather unexpectedly, representatives of Desulfovibrionaceae and Campylobacteraceae , both capable of hydrogen utilisation in complex microbial communities, belonged among core microbiota families. Understanding the roles of individual population members in the total metabolism of the complex community may allow for interventions which might result in the replacement of Campylobacteraceae with Desulfovibrionaceae and a reduction of Campylobacter colonisation in broilers, carcasses, and consequently poultry meat products.",
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Videnska, P, Rahman, MM, Faldynova, M, Babak, V, Matulova, ME, Prukner-Radovcic, E, Krizek, I, Smole-Mozina, S, Kovac, J, Szmolka, A, Nagy, B, Sedlar, K, Cejkova, D & Rychlik, I 2014, 'Characterization of egg laying hen and broiler fecal microbiota in poultry farms in Croatia, Czech Republic, Hungary and Slovenia', PloS one, vol. 9, no. 10, e110076. https://doi.org/10.1371/journal.pone.0110076

Characterization of egg laying hen and broiler fecal microbiota in poultry farms in Croatia, Czech Republic, Hungary and Slovenia. / Videnska, Petra; Rahman, Md Masudur; Faldynova, Marcela; Babak, Vladimir; Matulova, Marta Elsheimer; Prukner-Radovcic, Estella; Krizek, Ivan; Smole-Mozina, Sonja; Kovac, Jasna; Szmolka, Ama; Nagy, Bela; Sedlar, Karel; Cejkova, Darina; Rychlik, Ivan.

In: PloS one, Vol. 9, No. 10, e110076, 16.10.2014.

Research output: Contribution to journalArticle

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T1 - Characterization of egg laying hen and broiler fecal microbiota in poultry farms in Croatia, Czech Republic, Hungary and Slovenia

AU - Videnska, Petra

AU - Rahman, Md Masudur

AU - Faldynova, Marcela

AU - Babak, Vladimir

AU - Matulova, Marta Elsheimer

AU - Prukner-Radovcic, Estella

AU - Krizek, Ivan

AU - Smole-Mozina, Sonja

AU - Kovac, Jasna

AU - Szmolka, Ama

AU - Nagy, Bela

AU - Sedlar, Karel

AU - Cejkova, Darina

AU - Rychlik, Ivan

PY - 2014/10/16

Y1 - 2014/10/16

N2 - Poultry meat is the most common protein source of animal origin for humans. However, intensive breeding of animals in confined spaces has led to poultry colonisation by microbiota with a zoonotic potential or encoding antibiotic resistances. In this study we were therefore interested in the prevalence of selected antibiotic resistance genes and microbiota composition in feces of egg laying hens and broilers originating from 4 different Central European countries determined by real-time PCR and 16S rRNA gene pyrosequencing, respectively. strA gene was present in 1 out of 10,000 bacteria. The prevalence of sul1, sul2 and tet(B) in poultry microbiota was approx. 6 times lower than that of the strA gene. tet(A) and cat were the least prevalent being present in around 3 out of 10,000,000 bacteria forming fecal microbiome. The core chicken fecal microbiota was formed by 26 different families. Rather unexpectedly, representatives of Desulfovibrionaceae and Campylobacteraceae , both capable of hydrogen utilisation in complex microbial communities, belonged among core microbiota families. Understanding the roles of individual population members in the total metabolism of the complex community may allow for interventions which might result in the replacement of Campylobacteraceae with Desulfovibrionaceae and a reduction of Campylobacter colonisation in broilers, carcasses, and consequently poultry meat products.

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