Characterization of extracellular proteases produced by four ericoid mycorrhizal fungi in pure culture and in symbiotic states

Wei Qiang Yang, Ming Tien, Barbara L. Goulart

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Extracellular proteases produced by four mycorrhizal fungi growing in pure culture were compared to those produced by their symbionts (mycorrhizal blueberry plant roots). The profiles of the extracellular proteases produced by these isolates in pure culture were different from those produced by the symbionts. This conclusion was further supported by the results from isoelectric focusing study of the protease produced by Sterile White II (H. ericae, UAMH 9264) and its symbiont. No protease band was observed in nonmycorrhizal control plants. Different extracellular proteases were also produced by two different highbush blueberry cultivars infected with the same native isolate, Oidiodendron maius (UAMH 9263). The pH optima for protease activity were different between pure cultured mycorrhizal fungi and their symbionts. These results suggest that extracellular protease production by the symbiont is at least partially host controlled. The results are discussed in relation to ecological aspects of mycorrhizal symbiosis.

Original languageEnglish (US)
Title of host publicationProceedings of the Eighth International Symposium on Vaccinium Culture
PublisherInternational Society for Horticultural Science
Pages403-410
Number of pages8
ISBN (Print)9066055693, 9789066055698
DOIs
StatePublished - Jan 1 2006

Publication series

NameActa Horticulturae
Volume715
ISSN (Print)0567-7572

Fingerprint

mycorrhizal fungi
proteinases
symbionts
Vaccinium corymbosum
isoelectric focusing
blueberries
symbiosis
cultivars

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Yang, W. Q., Tien, M., & Goulart, B. L. (2006). Characterization of extracellular proteases produced by four ericoid mycorrhizal fungi in pure culture and in symbiotic states. In Proceedings of the Eighth International Symposium on Vaccinium Culture (pp. 403-410). (Acta Horticulturae; Vol. 715). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2006.715.60
Yang, Wei Qiang ; Tien, Ming ; Goulart, Barbara L. / Characterization of extracellular proteases produced by four ericoid mycorrhizal fungi in pure culture and in symbiotic states. Proceedings of the Eighth International Symposium on Vaccinium Culture. International Society for Horticultural Science, 2006. pp. 403-410 (Acta Horticulturae).
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Yang, WQ, Tien, M & Goulart, BL 2006, Characterization of extracellular proteases produced by four ericoid mycorrhizal fungi in pure culture and in symbiotic states. in Proceedings of the Eighth International Symposium on Vaccinium Culture. Acta Horticulturae, vol. 715, International Society for Horticultural Science, pp. 403-410. https://doi.org/10.17660/ActaHortic.2006.715.60

Characterization of extracellular proteases produced by four ericoid mycorrhizal fungi in pure culture and in symbiotic states. / Yang, Wei Qiang; Tien, Ming; Goulart, Barbara L.

Proceedings of the Eighth International Symposium on Vaccinium Culture. International Society for Horticultural Science, 2006. p. 403-410 (Acta Horticulturae; Vol. 715).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Yang WQ, Tien M, Goulart BL. Characterization of extracellular proteases produced by four ericoid mycorrhizal fungi in pure culture and in symbiotic states. In Proceedings of the Eighth International Symposium on Vaccinium Culture. International Society for Horticultural Science. 2006. p. 403-410. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2006.715.60