Characterization of Fusobacterium isolates from the respiratory tract of white-tailed deer (Odocoileus virginianus)

Jason W. Brooks, Amit Kumar, Sanjeev Narayanan, Suzanne Myers, Kayla Brown, T. G. Nagaraja, Bhushan M. Jayarao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A total of 23 clinical isolates of Fusobacterium spp. were recovered at necropsy over a 2-year period from the respiratory tract of white-tailed deer (Odocoileus virginianus). Isolates were identified as Fusobacterium varium (18/23), Fusobacterium necrophorum subsp. funduliforme (3/23), and Fusobacterium necrophorum subsp. necrophorum (2/23). Using polymerase chain reaction-based detection of virulence genes, all F. necrophorum isolates were positive for the promoter region of the leukotoxin operon and the hemagglutinin-related protein gene, while all F. varium isolates were negative. The presence of the leukotoxin gene in F. necrophorum isolates and the absence of this gene in F. varium isolates were confirmed by Southern hybridization using 2 separate probes. Toxicity to bovine polymorphonuclear leukocytes was observed with all F. necrophorum isolates, but was not observed in any F. varium isolates. Susceptibility to antimicrobials was markedly different for F. varium as compared to F. necrophorum. In summary, no evidence of leukotoxin production was detected in any of the 23 F. varium isolates used in the current study. The data suggests that F. varium, the most common species isolated, may be a significant pathogen in deer with a different virulence mechanism than F. necrophorum.

Original languageEnglish (US)
Pages (from-to)213-220
Number of pages8
JournalJournal of Veterinary Diagnostic Investigation
Volume26
Issue number2
DOIs
StatePublished - Mar 2014

Fingerprint

Fusobacterium varium
Fusobacterium necrophorum
Fusobacterium
Deer
Odocoileus virginianus
respiratory system
Respiratory System
leukotoxins
Fusobacterium necrophorum subsp. funduliforme
Virulence
Fusobacterium necrophorum subsp. necrophorum
virulence
genes
Genes
Hemagglutinins
hemagglutinins
operon
Operon
Genetic Promoter Regions
Southern blotting

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

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title = "Characterization of Fusobacterium isolates from the respiratory tract of white-tailed deer (Odocoileus virginianus)",
abstract = "A total of 23 clinical isolates of Fusobacterium spp. were recovered at necropsy over a 2-year period from the respiratory tract of white-tailed deer (Odocoileus virginianus). Isolates were identified as Fusobacterium varium (18/23), Fusobacterium necrophorum subsp. funduliforme (3/23), and Fusobacterium necrophorum subsp. necrophorum (2/23). Using polymerase chain reaction-based detection of virulence genes, all F. necrophorum isolates were positive for the promoter region of the leukotoxin operon and the hemagglutinin-related protein gene, while all F. varium isolates were negative. The presence of the leukotoxin gene in F. necrophorum isolates and the absence of this gene in F. varium isolates were confirmed by Southern hybridization using 2 separate probes. Toxicity to bovine polymorphonuclear leukocytes was observed with all F. necrophorum isolates, but was not observed in any F. varium isolates. Susceptibility to antimicrobials was markedly different for F. varium as compared to F. necrophorum. In summary, no evidence of leukotoxin production was detected in any of the 23 F. varium isolates used in the current study. The data suggests that F. varium, the most common species isolated, may be a significant pathogen in deer with a different virulence mechanism than F. necrophorum.",
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Characterization of Fusobacterium isolates from the respiratory tract of white-tailed deer (Odocoileus virginianus). / Brooks, Jason W.; Kumar, Amit; Narayanan, Sanjeev; Myers, Suzanne; Brown, Kayla; Nagaraja, T. G.; Jayarao, Bhushan M.

In: Journal of Veterinary Diagnostic Investigation, Vol. 26, No. 2, 03.2014, p. 213-220.

Research output: Contribution to journalArticle

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