Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

Karen Dohmann, Birgit Strommenger, Karen Stevenson, Lucía De Juan, Janin Stratmann, Vivek Kapur, Tim J. Bull, Gerald Friedrich Gerlach

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

A combination of representational difference analysis and comparative DNA sequencing revealed that tour type I (sheep) isolates of Mycobacterium avium subsp. paratuberculosis were differentiated from nine type II (bovine) isolates by the presence of an ll-bp insertion in a novel M. avium subsp. paratuberculosis-specific region of genomic DNA. Further, our studies show that M. avium subsp. paratuberculosis type I isolates contain three type-specific loci that are missing in M. avium subsp. paratuberculosis type II but are present in M. avium subsp. avium. Taken together, the results are consistent with the hypothesis that M. avium subsp. paratuberculosis type I strains are an evolutionary intermediate between M. avium subsp. avium and M. avium subsp. paratuberculosis type II isolates or share a subset of M. avium subsp. avium type-specific loci through horizontal transfer.

Original languageEnglish (US)
Pages (from-to)5215-5223
Number of pages9
JournalJournal of clinical microbiology
Volume41
Issue number11
DOIs
StatePublished - Nov 1 2003

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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