Characterization of human translesion DNA synthesis across a UV-induced DNA lesion

Mark Hedglin, Binod Pandey, Stephen Benkovic

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Translesion DNA synthesis (TLS) during S-phase uses specialized TLS DNA polymerases to replicate a DNA lesion, allowing stringent DNA synthesis to resume beyond the offending damage. Human TLS involves the conjugation of ubiquitin to PCNA clamps encircling damaged DNA and the role of this post-translational modification is under scrutiny. A widely-accepted model purports that ubiquitinated PCNA recruits TLS polymerases such as pol h to sites of DNA damage where they may also displace a blocked replicative polymerase. We provide extensive quantitative evidence that the binding of pol h to PCNA and the ensuing TLS are both independent of PCNA ubiquitination. Rather, the unique properties of pols h and d are attuned to promote an efficient and passive exchange of polymerases during TLS on the lagging strand.

Original languageEnglish (US)
Article numbere19788
JournaleLife
Volume5
Issue numberOCTOBER2016
DOIs
StatePublished - Oct 22 2016

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DNA
Proliferating Cell Nuclear Antigen
DNA-Directed DNA Polymerase
Ubiquitination
Post Translational Protein Processing
Ubiquitin
S Phase
DNA Damage
Clamping devices
Ion exchange

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Hedglin, Mark ; Pandey, Binod ; Benkovic, Stephen. / Characterization of human translesion DNA synthesis across a UV-induced DNA lesion. In: eLife. 2016 ; Vol. 5, No. OCTOBER2016.
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Characterization of human translesion DNA synthesis across a UV-induced DNA lesion. / Hedglin, Mark; Pandey, Binod; Benkovic, Stephen.

In: eLife, Vol. 5, No. OCTOBER2016, e19788, 22.10.2016.

Research output: Contribution to journalArticle

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