Sera obtained from normal B10.BR mice were shown to inhibit selectively a specific anti Ia alloantiserum. Partial purification of the Ia antigenic activity was accomplished by isolation of the high density lipoproteins from these sera by fractional precipitation with sodium phosphotungstate and MgCl 2. Both H 2.23 and Ia(k) antigens present in this high density lipoprotein fraction were completely adsorbed by rabbit anti rat β 2 microglobulin immunoadsorbents, whereas specific anti H 2.23 immunoadsorbents removed only the H 2 activity. These data demonstrate that Ia antigens, like H 2 antigens in the sera of B10.BR mice are associated with high density lipoproteins and further suggest that both H 2 and Ia antigens are associated with a β 2 microglobulin like molecule.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Immunology|
|State||Published - Dec 1 1976|
All Science Journal Classification (ASJC) codes
- Immunology and Allergy