TY - JOUR
T1 - Characterization of lipid-linked octa-, nona-, and decasaccharides formed during in vitro synthesis of mammary glycoproteins
AU - Vijay, Inder K.
AU - Perdew, Gary H.
N1 - Funding Information:
i This work was supported by grant AM19682 and Research Career Development Award AM452 from the National Institutes of Health to I.K.V. Contribution No. A-3072 of the Maryland Agricultural Experiment Station, Scientific Article #6137 of the Department of Animal Sciences. ’ Author to whom all correspondence should be addressed. 3 Abbreviations used: GlcNAc, N-aeetylglucosa-mine; (GlcNAc),, N,W-diacetylchitobiose; GleNAeon, N-acetylglucosaminitol; GlcNAco,, N-acetyl-[3Hjglucosaminitol; Man, mannose; Glc, glucose; endo, endo-P-N-acetylglucosaminidase; saccharide III, IV, V XI refer to products identified as tri-, tetra-, penta-, and undecasaccharide; CHO cells, Chinese hamster ovary cells; DTT, dithiothreitol.
PY - 1983/2/1
Y1 - 1983/2/1
N2 - The lipid-linked octa-, nona-, and decasaccharides, isolated from incubations of a membrane preparation from the lactating bovine mammary tissue with GDP-[14C]mannose and UDP-N-acetylglucosamine were subjected to mild acid hydrolysis and purified extensively by repeated gel filtration and paper chromatography. Structural characterization of the oligosaccharides containing six to eight mannose residues linked to an N,N′-diacetylchitobiose unit utilizing digestions with α-mannosidase, β-mannosidase, endo-β-N-acetylglucosaminidase, D, H, and L, acetolysis, and methylation analysis revealed the presence of several isomers within each size species. Supplementation of the incubations with 0.1 mm dolichol phosphate reduces the number of isomers within these oligosaccharides; the predominant isomers of saccharides from these incubations appear to be similar to the saccharides isolated from in vivo preparations of Chinese hamster ovary cells.
AB - The lipid-linked octa-, nona-, and decasaccharides, isolated from incubations of a membrane preparation from the lactating bovine mammary tissue with GDP-[14C]mannose and UDP-N-acetylglucosamine were subjected to mild acid hydrolysis and purified extensively by repeated gel filtration and paper chromatography. Structural characterization of the oligosaccharides containing six to eight mannose residues linked to an N,N′-diacetylchitobiose unit utilizing digestions with α-mannosidase, β-mannosidase, endo-β-N-acetylglucosaminidase, D, H, and L, acetolysis, and methylation analysis revealed the presence of several isomers within each size species. Supplementation of the incubations with 0.1 mm dolichol phosphate reduces the number of isomers within these oligosaccharides; the predominant isomers of saccharides from these incubations appear to be similar to the saccharides isolated from in vivo preparations of Chinese hamster ovary cells.
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U2 - 10.1016/0003-9861(83)90453-8
DO - 10.1016/0003-9861(83)90453-8
M3 - Article
C2 - 6824342
AN - SCOPUS:0020622246
VL - 220
SP - 605
EP - 614
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 2
ER -