TY - JOUR
T1 - Characterization of macromolecular orientation in κ-carrageenan fibers using polarized Fourier-transform infrared spectroscopy
AU - Kong, Lingyan
AU - Stapleton, Joshua J.
AU - Ziegler, Gregory R.
N1 - Funding Information:
This work was supported by the USDA National Institute for Food and Agriculture , National Competitive Grants Program , National Research Initiative Program 71.1 FY 2007 as grant # 2007–35503-18392. The authors thank Dr. Trevor Clark for sample preparation with focused ion beam. The authors also thank Dr. Srinivas Janaswamy for assistance with X-ray fiber diffraction. Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Basic Energy Sciences, Office of Science , under Contract No. DE-AC02-06CH11357. Use of BioCARS was also supported by the National Institute of General Medical Sciences of the National Institutes of Health under grant number R24GM111072. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/1
Y1 - 2018/1
N2 - In the current study, polarized infrared (IR) microspectroscopy was employed to characterize the macromolecular orientation in wet-spun and stretched κ-carrageenan fibers. The fibers were shown to be well oriented by X-ray diffraction, suggesting that the κ-carrageenan molecules were generally aligned along the fiber axis direction. Longitudinal fiber pieces of about 10 μm thick were obtained by focused ion beam (FIB) micromilling. The fiber pieces were examined by polarized IR in transmission mode. Several bands, including those characteristic of κ-carrageenan at 845 and 930 cm−1, were polarization-dependent, demonstrating polarized IR as a useful tool to evaluate macromolecular orientation in carrageenan fibers. Band assignments were discussed by considering the general alignment of molecules and the polarization dependence of vibration modes, and our results agreed well with band assignments from previous reports.
AB - In the current study, polarized infrared (IR) microspectroscopy was employed to characterize the macromolecular orientation in wet-spun and stretched κ-carrageenan fibers. The fibers were shown to be well oriented by X-ray diffraction, suggesting that the κ-carrageenan molecules were generally aligned along the fiber axis direction. Longitudinal fiber pieces of about 10 μm thick were obtained by focused ion beam (FIB) micromilling. The fiber pieces were examined by polarized IR in transmission mode. Several bands, including those characteristic of κ-carrageenan at 845 and 930 cm−1, were polarization-dependent, demonstrating polarized IR as a useful tool to evaluate macromolecular orientation in carrageenan fibers. Band assignments were discussed by considering the general alignment of molecules and the polarization dependence of vibration modes, and our results agreed well with band assignments from previous reports.
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U2 - 10.1016/j.vibspec.2017.12.004
DO - 10.1016/j.vibspec.2017.12.004
M3 - Article
AN - SCOPUS:85038818526
VL - 94
SP - 61
EP - 65
JO - Vibrational Spectroscopy
JF - Vibrational Spectroscopy
SN - 0924-2031
ER -