TY - JOUR
T1 - Characterization of Maize Near-Isogenic Lines With Enhanced Flavonoid Expression to Be Used as Tools in Diet-Health Complexity
AU - Wu, Binning
AU - Chang, Haotian
AU - Marini, Rich
AU - Chopra, Surinder
AU - Reddivari, Lavanya
N1 - Funding Information:
The authors acknowledge Dr. Lori A. Hoagland for reviewing this manuscript. The authors thank Dr. Iffa Gaffoor for assistance with maize crosses during the early stages of development of near-isogenic lines, Dr. Kameron Wittmeyer and Debamalya Chatterjee for their advice on maize kernel dissection and flavonoid extractions, Dr. Jin Cui for help with pollinations and Penn State Agronomy farm staff for assistance with the tending of maize crop. The authors also thank Dr. Bruce Cooper and Amber Jannasch from Purdue University Metabolite Profiling Facility for assistance with LC-MS/MS and metabolomics data analysis. Funding. This research was funded by USDA-NIFA awards 2016-67017-29285 to LR, 2019-67017-29258 to LR and SC, and 2011-67009-30017 and 2019-70006-30442 to SC. Hatch funding PEN04613 to SC is acknowledged.
PY - 2021/1/18
Y1 - 2021/1/18
N2 - Increasing incidence of chronic diseases in the 21st century has emphasized the importance of developing crops with enhanced nutritional value. Plant-based diets are associated with reduced incidence of many chronic diseases. The growing population and increased food demand have prioritized the development of high-yielding commercial crop varieties at the expense of natural flavors as well as health-benefiting compounds including polyphenols. Flavonoids are a large subfamily of polyphenols abundant in the plant kingdom with known health-promoting effects, making them a promising trait to be re-introduced into elite lines. Given the vast array of flavonoids and the complexity of plant food metabolome interactions, it is difficult to identify with certainty the specific class(es) of flavonoids in the food matrix that are anti-inflammatory. To address this, we have developed four maize near-isogenic lines (NILs); a line that lacked both anthocyanins and phlobaphenes, a second NIL containing phlobaphenes, a third line had anthocyanins, and a fourth line that contained both anthocyanins and phlobaphenes. The phytochemical profiles and the antioxidant potential of the NILs were characterized. The accumulation of anthocyanins and phlobaphenes contributed significantly to antioxidant capacity compared to maize lines that lacked one or both of the compounds (p < 0.05). Pilot study showed that intake of flavonoid-rich maize diets were able to alleviate experimental colitis in mice. These NILs offer novel materials combining anthocyanins and phlobaphenes and can be used as powerful tools to investigate the disease-preventive effects of specific flavonoid compound in diet/feeding experiments.
AB - Increasing incidence of chronic diseases in the 21st century has emphasized the importance of developing crops with enhanced nutritional value. Plant-based diets are associated with reduced incidence of many chronic diseases. The growing population and increased food demand have prioritized the development of high-yielding commercial crop varieties at the expense of natural flavors as well as health-benefiting compounds including polyphenols. Flavonoids are a large subfamily of polyphenols abundant in the plant kingdom with known health-promoting effects, making them a promising trait to be re-introduced into elite lines. Given the vast array of flavonoids and the complexity of plant food metabolome interactions, it is difficult to identify with certainty the specific class(es) of flavonoids in the food matrix that are anti-inflammatory. To address this, we have developed four maize near-isogenic lines (NILs); a line that lacked both anthocyanins and phlobaphenes, a second NIL containing phlobaphenes, a third line had anthocyanins, and a fourth line that contained both anthocyanins and phlobaphenes. The phytochemical profiles and the antioxidant potential of the NILs were characterized. The accumulation of anthocyanins and phlobaphenes contributed significantly to antioxidant capacity compared to maize lines that lacked one or both of the compounds (p < 0.05). Pilot study showed that intake of flavonoid-rich maize diets were able to alleviate experimental colitis in mice. These NILs offer novel materials combining anthocyanins and phlobaphenes and can be used as powerful tools to investigate the disease-preventive effects of specific flavonoid compound in diet/feeding experiments.
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U2 - 10.3389/fpls.2020.619598
DO - 10.3389/fpls.2020.619598
M3 - Article
C2 - 33584759
AN - SCOPUS:85100572307
VL - 11
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 619598
ER -