Characterization of meiotic crossovers and gene conversion by whole-genome sequencing in Saccharomyces cerevisiae

Ji Qi, Asela J. Wijeratne, Lynn P. Tomsho, Yi Hu, Stephan C. Schuster, Hong Ma

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Meiotic recombination alters frequency and distribution of genetic variation, impacting genetics and evolution. In the budding yeast, DNA double strand breaks (DSBs) and D loops form either crossovers (COs) or non-crossovers (NCOs), which occur at many sites in the genome. Differences at the nucleotide level associated with COs and NCOs enable us to detect these recombination events and their distributions. Results: We used high throughput sequencing to uncover over 46 thousand single nucleotide polymorphisms (SNPs) between two budding yeast strains and investigated meiotic recombinational events. We provided a detailed analysis of CO and NCO events, including number, size range, and distribution on chromosomes. We have detected 91 COs, very close to the average number from previous genetic studies, as well as 21 NCO events and mapped the positions of these events with high resolution. We have obtained DNA sequence-level evidence for a wide range of sizes of chromosomal regions involved in CO and NCO events. We show that a large fraction of the COs are accompanied by gene conversion (GC), indicating that meiotic recombination changes allelic frequencies, in addition to redistributing existing genetic variations. Conclusion: This work is the first reported study of meiotic recombination using high throughput sequencing technologies. Our results show that high-throughput sequencing is a sensitive method to uncover at single-base resolution details of CO and NCO events, including some complex patterns, providing new clues about the mechanism of this fundamental process.

Original languageEnglish (US)
Article number1471
Number of pages1
JournalBMC genomics
Volume10
DOIs
StatePublished - Oct 15 2009

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Gene Conversion
Genetic Recombination
Saccharomyces cerevisiae
Genome
Saccharomycetales
Molecular Evolution
Double-Stranded DNA Breaks
Single Nucleotide Polymorphism
Nucleotides
Chromosomes
Technology

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Genetics

Cite this

Qi, Ji ; Wijeratne, Asela J. ; Tomsho, Lynn P. ; Hu, Yi ; Schuster, Stephan C. ; Ma, Hong. / Characterization of meiotic crossovers and gene conversion by whole-genome sequencing in Saccharomyces cerevisiae. In: BMC genomics. 2009 ; Vol. 10.
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Characterization of meiotic crossovers and gene conversion by whole-genome sequencing in Saccharomyces cerevisiae. / Qi, Ji; Wijeratne, Asela J.; Tomsho, Lynn P.; Hu, Yi; Schuster, Stephan C.; Ma, Hong.

In: BMC genomics, Vol. 10, 1471, 15.10.2009.

Research output: Contribution to journalArticle

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