Characterization of microbial dysbiosis and metabolomic changes in dogs with acute diarrhea

Blake C. Guard, James W. Barr, Lavanya Reddivari, Cory Klemashevich, Arul Jayaraman, Jörg M. Steiner, Jairam Vanamala, Jan S. Suchodolski

Research output: Contribution to journalArticle

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Abstract

Limited information is available regarding the metabolic consequences of intestinal dysbiosis in dogs with acute onset of diarrhea. The aim of this study was to evaluate the fecal microbiome, fecal concentrations of short-chain fatty acids (SCFAs), as well as serum and urine metabolites in healthy dogs (n=13) and dogs with acute diarrhea (n=13). The fecal microbiome, SCFAs, and serum/urine metabolite profiles were characterized by 454-pyrosequencing of the 16S rRNA genes, GC/MS, and untargeted and targeted metabolomics approach using UPLC/MS and HPLC/MS, respectively. Significantly lower bacterial diversity was observed in dogs with acute diarrhea in regards to species richness, chao1, and Shannon index (p=0.0218, 0.0176, and 0.0033; respectively). Dogs with acute diarrhea had significantly different microbial communities compared to healthy dogs (unweighted Unifrac distances, ANOSIM p=0.0040). While Bacteroidetes, Faecalibacterium, and an unclassified genus within Ruminococcaceae were underrepresented, the genus Clostridium was overrepresented in dogs with acute diarrhea. Concentrations of fecal propionic acid were significantly decreased in acute diarrhea (p=0.0033), and were correlated to a decrease in Faecalibacterium (ρ=0.6725, p=0.0332). The predicted functional gene content of the microbiome (PICRUSt) revealed overrepresentations of genes for transposase enzymes as well as methyl accepting chemotaxis proteins in acute diarrhea. Serum concentrations of kynurenic acid and urine concentrations of 2-methyl-1H-indole and 5-Methoxy-1H-indole-3-carbaldehyde were significantly decreased in acute diarrhea (p=0.0048, 0.0185, and 0.0330, respectively). These results demonstrate that the fecal dysbiosis present in acute diarrhea is associated with altered systemic metabolic states.

Original languageEnglish (US)
Article numbere0127259
JournalPloS one
Volume10
Issue number5
DOIs
StatePublished - May 22 2015

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Dysbiosis
Metabolomics
metabolomics
Diarrhea
diarrhea
Volatile Fatty Acids
Genes
Dogs
Metabolites
dogs
Kynurenic Acid
Transposases
Clostridium
Microbiota
urine
Urine
short chain fatty acids
indoles
Enzymes
Serum

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Guard, B. C., Barr, J. W., Reddivari, L., Klemashevich, C., Jayaraman, A., Steiner, J. M., ... Suchodolski, J. S. (2015). Characterization of microbial dysbiosis and metabolomic changes in dogs with acute diarrhea. PloS one, 10(5), [e0127259]. https://doi.org/10.1371/journal.pone.0127259
Guard, Blake C. ; Barr, James W. ; Reddivari, Lavanya ; Klemashevich, Cory ; Jayaraman, Arul ; Steiner, Jörg M. ; Vanamala, Jairam ; Suchodolski, Jan S. / Characterization of microbial dysbiosis and metabolomic changes in dogs with acute diarrhea. In: PloS one. 2015 ; Vol. 10, No. 5.
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Guard, BC, Barr, JW, Reddivari, L, Klemashevich, C, Jayaraman, A, Steiner, JM, Vanamala, J & Suchodolski, JS 2015, 'Characterization of microbial dysbiosis and metabolomic changes in dogs with acute diarrhea', PloS one, vol. 10, no. 5, e0127259. https://doi.org/10.1371/journal.pone.0127259

Characterization of microbial dysbiosis and metabolomic changes in dogs with acute diarrhea. / Guard, Blake C.; Barr, James W.; Reddivari, Lavanya; Klemashevich, Cory; Jayaraman, Arul; Steiner, Jörg M.; Vanamala, Jairam; Suchodolski, Jan S.

In: PloS one, Vol. 10, No. 5, e0127259, 22.05.2015.

Research output: Contribution to journalArticle

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Guard BC, Barr JW, Reddivari L, Klemashevich C, Jayaraman A, Steiner JM et al. Characterization of microbial dysbiosis and metabolomic changes in dogs with acute diarrhea. PloS one. 2015 May 22;10(5). e0127259. https://doi.org/10.1371/journal.pone.0127259