Characterization of mtDNA SNP typing using quantitative real-time PCR with special emphasis on heteroplasmy detection and mixture ratio assessment

H. Niederstätter, M. D. Coble, Thomas John Parsons, W. Parson

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Using a plasmid DNA model and total genomic DNA (gDNA) reference samples, we examined the performance and forensic utility of a quantitative allele-discriminatory real-time PCR assay relying on TaqMan probes targeting the mitochondrial single nucleotide polymorphism (mtSNP) 16519T/C. For allele-calling and allele proportion assessment we used absolute quantification of both alleles as well as relative quantification based on endpoint fluorescence.

Original languageEnglish (US)
Pages (from-to)1-3
Number of pages3
JournalInternational Congress Series
Volume1288
DOIs
StatePublished - Apr 1 2006

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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