Characterization of novel akermanite: Poly-ε{lunate}-caprolactone scaffolds for human adipose-derived stem cells bone tissue engineering

A. S. Zanetti, G. T. Mccandless, J. Y. Chan, J. M. Gimble, Daniel J. Hayes

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In this study, three different akermanite:poly-ε{lunate}-caprolactone (PCL) composite scaffolds (wt%: 75:25, 50:50, 25:75) were characterized in terms of structure, compression strength, degradation rate and in vitro biocompatibility to human adipose-derived stem cells (hASC). Pure ceramic scaffolds [CellCeramTM, custom-made, 40:60wt%; β-tricalcium phosphate (β-TCP):hydroxyapatite (HA); and akermanite] and PCL scaffolds served as experimental controls. Compared to ceramic scaffolds, the authors hypothesized that optimal akermanite:PCL composites would have improved compression strength and comparable biocompatibility to hASC. Electron microscopy analysis revealed that PCL-containing scaffolds had the highest porosity but CellCeramTM had the greatest pore size. In general, compression strength in PCL-containing scaffolds was greater than in ceramic scaffolds. PCL-containing scaffolds were also more stable in culture than ceramic scaffolds. Nonetheless, mass losses after 21days were observed in all scaffold types. Reduced hASC metabolic activity and increased cell detachment were observed after acute exposure to akermanite:PCL extracts (wt%: 75:25, 50:50). Among the PCL-containing scaffolds, hASC cultured for 21days on akermanite:PCL (wt%: 75:25) discs displayed the highest viability, increased expression of osteogenic markers (alkaline phosphatase and osteocalcin) and lowest IL-6 expression. Together, the results indicate that akermanite:PCL composites may have appropriate mechanical and biocompatibility properties for use as bone tissue scaffolds.

Original languageEnglish (US)
Pages (from-to)389-404
Number of pages16
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume9
Issue number4
DOIs
StatePublished - Apr 1 2015

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Tissue Engineering
Scaffolds (biology)
Stem cells
Tissue engineering
Scaffolds
Bone
Stem Cells
Ceramics
Bone and Bones
Biocompatibility
Tissue Scaffolds
Porosity
Osteocalcin
Durapatite
Composite materials
Alkaline Phosphatase
Compaction
polycaprolactone
Glass ceramics
Interleukin-6

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

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title = "Characterization of novel akermanite: Poly-ε{lunate}-caprolactone scaffolds for human adipose-derived stem cells bone tissue engineering",
abstract = "In this study, three different akermanite:poly-ε{lunate}-caprolactone (PCL) composite scaffolds (wt{\%}: 75:25, 50:50, 25:75) were characterized in terms of structure, compression strength, degradation rate and in vitro biocompatibility to human adipose-derived stem cells (hASC). Pure ceramic scaffolds [CellCeramTM, custom-made, 40:60wt{\%}; β-tricalcium phosphate (β-TCP):hydroxyapatite (HA); and akermanite] and PCL scaffolds served as experimental controls. Compared to ceramic scaffolds, the authors hypothesized that optimal akermanite:PCL composites would have improved compression strength and comparable biocompatibility to hASC. Electron microscopy analysis revealed that PCL-containing scaffolds had the highest porosity but CellCeramTM had the greatest pore size. In general, compression strength in PCL-containing scaffolds was greater than in ceramic scaffolds. PCL-containing scaffolds were also more stable in culture than ceramic scaffolds. Nonetheless, mass losses after 21days were observed in all scaffold types. Reduced hASC metabolic activity and increased cell detachment were observed after acute exposure to akermanite:PCL extracts (wt{\%}: 75:25, 50:50). Among the PCL-containing scaffolds, hASC cultured for 21days on akermanite:PCL (wt{\%}: 75:25) discs displayed the highest viability, increased expression of osteogenic markers (alkaline phosphatase and osteocalcin) and lowest IL-6 expression. Together, the results indicate that akermanite:PCL composites may have appropriate mechanical and biocompatibility properties for use as bone tissue scaffolds.",
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Characterization of novel akermanite : Poly-ε{lunate}-caprolactone scaffolds for human adipose-derived stem cells bone tissue engineering. / Zanetti, A. S.; Mccandless, G. T.; Chan, J. Y.; Gimble, J. M.; Hayes, Daniel J.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 9, No. 4, 01.04.2015, p. 389-404.

Research output: Contribution to journalArticle

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T2 - Poly-ε{lunate}-caprolactone scaffolds for human adipose-derived stem cells bone tissue engineering

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AU - Hayes, Daniel J.

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