Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred

David R. Holding, Brenda G. Hunter, John P. Klingler, Song Wu, Xiaomei Guo, Bryan C. Gibbon, Rongling Wu, Jan Michele Schulze, Rudolf Jung, Brian A. Larkins

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Quality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway.

Original languageEnglish (US)
Pages (from-to)783-794
Number of pages12
JournalTheoretical And Applied Genetics
Volume122
Issue number4
DOIs
StatePublished - Mar 1 2011

Fingerprint

modifiers (genes)
inbred lines
Zea mays
quantitative trait loci
corn
Endosperm
Genes
Proteins
genes
proteins
endosperm
pyrophosphate-fructose 6-phosphate 1-phosphotransferase
6-phosphofructokinase
Modifier Genes
mutants
Genetic Linkage
pyrophosphates
Hardness
glycolysis
regulator genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Holding, David R. ; Hunter, Brenda G. ; Klingler, John P. ; Wu, Song ; Guo, Xiaomei ; Gibbon, Bryan C. ; Wu, Rongling ; Schulze, Jan Michele ; Jung, Rudolf ; Larkins, Brian A. / Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred. In: Theoretical And Applied Genetics. 2011 ; Vol. 122, No. 4. pp. 783-794.
@article{fc20955d26394a2eaa7bab85ba0d81a1,
title = "Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred",
abstract = "Quality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway.",
author = "Holding, {David R.} and Hunter, {Brenda G.} and Klingler, {John P.} and Song Wu and Xiaomei Guo and Gibbon, {Bryan C.} and Rongling Wu and Schulze, {Jan Michele} and Rudolf Jung and Larkins, {Brian A.}",
year = "2011",
month = "3",
day = "1",
doi = "10.1007/s00122-010-1486-3",
language = "English (US)",
volume = "122",
pages = "783--794",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "4",

}

Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred. / Holding, David R.; Hunter, Brenda G.; Klingler, John P.; Wu, Song; Guo, Xiaomei; Gibbon, Bryan C.; Wu, Rongling; Schulze, Jan Michele; Jung, Rudolf; Larkins, Brian A.

In: Theoretical And Applied Genetics, Vol. 122, No. 4, 01.03.2011, p. 783-794.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred

AU - Holding, David R.

AU - Hunter, Brenda G.

AU - Klingler, John P.

AU - Wu, Song

AU - Guo, Xiaomei

AU - Gibbon, Bryan C.

AU - Wu, Rongling

AU - Schulze, Jan Michele

AU - Jung, Rudolf

AU - Larkins, Brian A.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Quality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway.

AB - Quality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway.

UR - http://www.scopus.com/inward/record.url?scp=79952443826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952443826&partnerID=8YFLogxK

U2 - 10.1007/s00122-010-1486-3

DO - 10.1007/s00122-010-1486-3

M3 - Article

VL - 122

SP - 783

EP - 794

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 4

ER -