Characterization of protein kinases in mitotic and meiotic cell extracts

Margaret S. Halleck, Katherine Lumley-Sapanski, Jon A. Reed, Anand P. Iyer, Andrea M. Mastro, Robert A. Schlegel

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A number of protein kinases have been separated and identified in extracts from mitotic and interphase culture cells and from mature and immature amphibian oocytes using nondenaturing polyacrylamide gel electrophoresis followed by in situ phosphorylation assays. Certain of these protein kinase activities appear to correlate with the biological activity of extracts, assayed by their ability to induce meiotic maturation following injection into Xenopus oocytes. These results are consistent with the notion that protein phosphorylation/dephosphorylation may be integral to the mechanisms of both nuclear membrane breakdown and chromosome condensation, events common and distinctive to mitosis and meiosis.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalFEBS Letters
Volume167
Issue number2
DOIs
StatePublished - Feb 27 1984

Fingerprint

Phosphorylation
Cell Extracts
Protein Kinases
Oocytes
Interphase
Nuclear Envelope
Meiosis
Amphibians
Chromosomes
Xenopus
Bioactivity
Electrophoresis
Cell culture
Mitosis
Polyacrylamide Gel Electrophoresis
Condensation
Assays
Cell Culture Techniques
Membranes
Injections

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Halleck, M. S., Lumley-Sapanski, K., Reed, J. A., Iyer, A. P., Mastro, A. M., & Schlegel, R. A. (1984). Characterization of protein kinases in mitotic and meiotic cell extracts. FEBS Letters, 167(2), 193-198. https://doi.org/10.1016/0014-5793(84)80125-8
Halleck, Margaret S. ; Lumley-Sapanski, Katherine ; Reed, Jon A. ; Iyer, Anand P. ; Mastro, Andrea M. ; Schlegel, Robert A. / Characterization of protein kinases in mitotic and meiotic cell extracts. In: FEBS Letters. 1984 ; Vol. 167, No. 2. pp. 193-198.
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Halleck, MS, Lumley-Sapanski, K, Reed, JA, Iyer, AP, Mastro, AM & Schlegel, RA 1984, 'Characterization of protein kinases in mitotic and meiotic cell extracts', FEBS Letters, vol. 167, no. 2, pp. 193-198. https://doi.org/10.1016/0014-5793(84)80125-8

Characterization of protein kinases in mitotic and meiotic cell extracts. / Halleck, Margaret S.; Lumley-Sapanski, Katherine; Reed, Jon A.; Iyer, Anand P.; Mastro, Andrea M.; Schlegel, Robert A.

In: FEBS Letters, Vol. 167, No. 2, 27.02.1984, p. 193-198.

Research output: Contribution to journalArticle

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Halleck MS, Lumley-Sapanski K, Reed JA, Iyer AP, Mastro AM, Schlegel RA. Characterization of protein kinases in mitotic and meiotic cell extracts. FEBS Letters. 1984 Feb 27;167(2):193-198. https://doi.org/10.1016/0014-5793(84)80125-8