Characterization of the acute lactational response to trans-10, cis-12 conjugated linoleic acid

Kevin John Harvatine, D. E. Bauman

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Trans-10, cis-12 conjugated linoleic acid (CLA) is a potent inhibitor of milk fat synthesis in the dairy cow. The decrease in milk fat yield during abomasal infusion of CLA reaches a nadir after 3 to 5 d. The acute responses to CLA were evaluated using 4 cows in a crossover design. Cows were milked with the aid of oxytocin every 4. h from -28 to 80. h and every 6. h from 86 to 116. h relative to the initiation of abomasal CLA infusion. An initial priming dose of 7.5. g of CLA was given at time zero followed by infusion of 2.5. g every 4. h for 72. h. Plasma CLA reached a near-steady-state concentration by 4. h, and initial plasma enrichments were greatest in the triglyceride and nonesterified fatty acid fractions. Milk CLA concentration peaked at 6. h and reached steady state by 22. h. At termination of the infusion, decreases in milk CLA concentration and yield and plasma CLA concentration were best fit by a reciprocal-linear function. Milk fat percentage decreased progressively after 2. h and was significant by 14. h. Milk fatty acid profile was initially unchanged, but between 18 and 36. h after initiation of the CLA dose the proportions of fatty acids progressively shifted, resulting in an increase in fatty acids >C16 and a decrease in fatty acids <C16 by 38 to 46. h. In contrast, changes in the desaturase index were immediate, with a significant decrease by 6. h and a near-maximal decrease by 10. h. Thus, stearoyl desaturase enzyme was more acutely responsive to CLA than other enzymes in milk fat synthesis. The initial decrease in milk fat synthesis involved an equal depression of short- and long-chain fatty acid pathways and was followed thereafter by a more pronounced decrease in the synthesis of de novo fatty acids.

Original languageEnglish (US)
Pages (from-to)6047-6056
Number of pages10
JournalJournal of dairy science
Volume94
Issue number12
DOIs
StatePublished - Dec 1 2011

Fingerprint

Conjugated Linoleic Acids
conjugated linoleic acid
Milk
Fatty Acids
Fats
milk fat
fatty acids
synthesis
trans-10,cis-12-conjugated linoleic acid
cows
milk
milk fat percentage
Oxytocin
Enzymes
milk fat yield
oxytocin
long chain fatty acids
Nonesterified Fatty Acids
dosage
Cross-Over Studies

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

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abstract = "Trans-10, cis-12 conjugated linoleic acid (CLA) is a potent inhibitor of milk fat synthesis in the dairy cow. The decrease in milk fat yield during abomasal infusion of CLA reaches a nadir after 3 to 5 d. The acute responses to CLA were evaluated using 4 cows in a crossover design. Cows were milked with the aid of oxytocin every 4. h from -28 to 80. h and every 6. h from 86 to 116. h relative to the initiation of abomasal CLA infusion. An initial priming dose of 7.5. g of CLA was given at time zero followed by infusion of 2.5. g every 4. h for 72. h. Plasma CLA reached a near-steady-state concentration by 4. h, and initial plasma enrichments were greatest in the triglyceride and nonesterified fatty acid fractions. Milk CLA concentration peaked at 6. h and reached steady state by 22. h. At termination of the infusion, decreases in milk CLA concentration and yield and plasma CLA concentration were best fit by a reciprocal-linear function. Milk fat percentage decreased progressively after 2. h and was significant by 14. h. Milk fatty acid profile was initially unchanged, but between 18 and 36. h after initiation of the CLA dose the proportions of fatty acids progressively shifted, resulting in an increase in fatty acids >C16 and a decrease in fatty acids <C16 by 38 to 46. h. In contrast, changes in the desaturase index were immediate, with a significant decrease by 6. h and a near-maximal decrease by 10. h. Thus, stearoyl desaturase enzyme was more acutely responsive to CLA than other enzymes in milk fat synthesis. The initial decrease in milk fat synthesis involved an equal depression of short- and long-chain fatty acid pathways and was followed thereafter by a more pronounced decrease in the synthesis of de novo fatty acids.",
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Characterization of the acute lactational response to trans-10, cis-12 conjugated linoleic acid. / Harvatine, Kevin John; Bauman, D. E.

In: Journal of dairy science, Vol. 94, No. 12, 01.12.2011, p. 6047-6056.

Research output: Contribution to journalArticle

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