Abstract
Trans-10, cis-12 conjugated linoleic acid (CLA) is a potent inhibitor of milk fat synthesis in the dairy cow. The decrease in milk fat yield during abomasal infusion of CLA reaches a nadir after 3 to 5 d. The acute responses to CLA were evaluated using 4 cows in a crossover design. Cows were milked with the aid of oxytocin every 4. h from -28 to 80. h and every 6. h from 86 to 116. h relative to the initiation of abomasal CLA infusion. An initial priming dose of 7.5. g of CLA was given at time zero followed by infusion of 2.5. g every 4. h for 72. h. Plasma CLA reached a near-steady-state concentration by 4. h, and initial plasma enrichments were greatest in the triglyceride and nonesterified fatty acid fractions. Milk CLA concentration peaked at 6. h and reached steady state by 22. h. At termination of the infusion, decreases in milk CLA concentration and yield and plasma CLA concentration were best fit by a reciprocal-linear function. Milk fat percentage decreased progressively after 2. h and was significant by 14. h. Milk fatty acid profile was initially unchanged, but between 18 and 36. h after initiation of the CLA dose the proportions of fatty acids progressively shifted, resulting in an increase in fatty acids >C16 and a decrease in fatty acids <C16 by 38 to 46. h. In contrast, changes in the desaturase index were immediate, with a significant decrease by 6. h and a near-maximal decrease by 10. h. Thus, stearoyl desaturase enzyme was more acutely responsive to CLA than other enzymes in milk fat synthesis. The initial decrease in milk fat synthesis involved an equal depression of short- and long-chain fatty acid pathways and was followed thereafter by a more pronounced decrease in the synthesis of de novo fatty acids.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6047-6056 |
| Number of pages | 10 |
| Journal | Journal of dairy science |
| Volume | 94 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1 2011 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Food Science
- Animal Science and Zoology
- Genetics
Cite this
}
Characterization of the acute lactational response to trans-10, cis-12 conjugated linoleic acid. / Harvatine, Kevin John; Bauman, D. E.
In: Journal of dairy science, Vol. 94, No. 12, 01.12.2011, p. 6047-6056.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterization of the acute lactational response to trans-10, cis-12 conjugated linoleic acid
AU - Harvatine, Kevin John
AU - Bauman, D. E.
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Trans-10, cis-12 conjugated linoleic acid (CLA) is a potent inhibitor of milk fat synthesis in the dairy cow. The decrease in milk fat yield during abomasal infusion of CLA reaches a nadir after 3 to 5 d. The acute responses to CLA were evaluated using 4 cows in a crossover design. Cows were milked with the aid of oxytocin every 4. h from -28 to 80. h and every 6. h from 86 to 116. h relative to the initiation of abomasal CLA infusion. An initial priming dose of 7.5. g of CLA was given at time zero followed by infusion of 2.5. g every 4. h for 72. h. Plasma CLA reached a near-steady-state concentration by 4. h, and initial plasma enrichments were greatest in the triglyceride and nonesterified fatty acid fractions. Milk CLA concentration peaked at 6. h and reached steady state by 22. h. At termination of the infusion, decreases in milk CLA concentration and yield and plasma CLA concentration were best fit by a reciprocal-linear function. Milk fat percentage decreased progressively after 2. h and was significant by 14. h. Milk fatty acid profile was initially unchanged, but between 18 and 36. h after initiation of the CLA dose the proportions of fatty acids progressively shifted, resulting in an increase in fatty acids >C16 and a decrease in fatty acids <C16 by 38 to 46. h. In contrast, changes in the desaturase index were immediate, with a significant decrease by 6. h and a near-maximal decrease by 10. h. Thus, stearoyl desaturase enzyme was more acutely responsive to CLA than other enzymes in milk fat synthesis. The initial decrease in milk fat synthesis involved an equal depression of short- and long-chain fatty acid pathways and was followed thereafter by a more pronounced decrease in the synthesis of de novo fatty acids.
AB - Trans-10, cis-12 conjugated linoleic acid (CLA) is a potent inhibitor of milk fat synthesis in the dairy cow. The decrease in milk fat yield during abomasal infusion of CLA reaches a nadir after 3 to 5 d. The acute responses to CLA were evaluated using 4 cows in a crossover design. Cows were milked with the aid of oxytocin every 4. h from -28 to 80. h and every 6. h from 86 to 116. h relative to the initiation of abomasal CLA infusion. An initial priming dose of 7.5. g of CLA was given at time zero followed by infusion of 2.5. g every 4. h for 72. h. Plasma CLA reached a near-steady-state concentration by 4. h, and initial plasma enrichments were greatest in the triglyceride and nonesterified fatty acid fractions. Milk CLA concentration peaked at 6. h and reached steady state by 22. h. At termination of the infusion, decreases in milk CLA concentration and yield and plasma CLA concentration were best fit by a reciprocal-linear function. Milk fat percentage decreased progressively after 2. h and was significant by 14. h. Milk fatty acid profile was initially unchanged, but between 18 and 36. h after initiation of the CLA dose the proportions of fatty acids progressively shifted, resulting in an increase in fatty acids >C16 and a decrease in fatty acids <C16 by 38 to 46. h. In contrast, changes in the desaturase index were immediate, with a significant decrease by 6. h and a near-maximal decrease by 10. h. Thus, stearoyl desaturase enzyme was more acutely responsive to CLA than other enzymes in milk fat synthesis. The initial decrease in milk fat synthesis involved an equal depression of short- and long-chain fatty acid pathways and was followed thereafter by a more pronounced decrease in the synthesis of de novo fatty acids.
UR - http://www.scopus.com/inward/record.url?scp=82155192895&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=82155192895&partnerID=8YFLogxK
U2 - 10.3168/jds.2011-4657
DO - 10.3168/jds.2011-4657
M3 - Article
C2 - 22118092
AN - SCOPUS:82155192895
VL - 94
SP - 6047
EP - 6056
JO - Journal of Dairy Science
JF - Journal of Dairy Science
SN - 0022-0302
IS - 12
ER -