Characterization of the basal angiosperm Aristolochia fimbriata: A potential experimental system for genetic studies

Barbara J. Bliss, Stefan Wanke, Abdelali Barakat, Saravanaraj Ayyampalayam, Norman Wickett, P. Kerr Wall, Yuannian Jiao, Lena Landherr, Paula E. Ralph, Yi Hu, Christoph Neinhuis, Jim Leebens-Mack, Kathiravetpilla Arumuganathan, Sandra W. Clifton, Siela N. Maximova, Hong Ma, Claude W. dePamphilis

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Abstract

Background: Previous studies in basal angiosperms have provided insight into the diversity within the angiosperm lineage and helped to polarize analyses of flowering plant evolution. However, there is still not an experimental system for genetic studies among basal angiosperms to facilitate comparative studies and functional investigation. It would be desirable to identify a basal angiosperm experimental system that possesses many of the features found in existing plant model systems (e.g., Arabidopsis and Oryza).Results: We have considered all basal angiosperm families for general characteristics important for experimental systems, including availability to the scientific community, growth habit, and membership in a large basal angiosperm group that displays a wide spectrum of phenotypic diversity. Most basal angiosperms are woody or aquatic, thus are not well-suited for large scale cultivation, and were excluded. We further investigated members of Aristolochiaceae for ease of culture, life cycle, genome size, and chromosome number. We demonstrated self-compatibility for Aristolochia elegans and A. fimbriata, and transformation with a GFP reporter construct for Saruma henryi and A. fimbriata. Furthermore, A. fimbriata was easily cultivated with a life cycle of just three months, could be regenerated in a tissue culture system, and had one of the smallest genomes among basal angiosperms. An extensive multi-tissue EST dataset was produced for A. fimbriata that includes over 3.8 million 454 sequence reads.Conclusions: Aristolochia fimbriata has numerous features that facilitate genetic studies and is suggested as a potential model system for use with a wide variety of technologies. Emerging genetic and genomic tools for A. fimbriata and closely related species can aid the investigation of floral biology, developmental genetics, biochemical pathways important in plant-insect interactions as well as human health, and various other features present in early angiosperms.

Original languageEnglish (US)
Article number13
JournalBMC plant biology
Volume13
Issue number1
DOIs
StatePublished - Jan 24 2013

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Aristolochia
Angiospermae
life cycle (organisms)
Aristolochiaceae
plant-insect relations
genome
Oryza
growth habit
chromosome number
tissue culture
human health
biochemical pathways
Arabidopsis

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Bliss, Barbara J. ; Wanke, Stefan ; Barakat, Abdelali ; Ayyampalayam, Saravanaraj ; Wickett, Norman ; Wall, P. Kerr ; Jiao, Yuannian ; Landherr, Lena ; Ralph, Paula E. ; Hu, Yi ; Neinhuis, Christoph ; Leebens-Mack, Jim ; Arumuganathan, Kathiravetpilla ; Clifton, Sandra W. ; Maximova, Siela N. ; Ma, Hong ; dePamphilis, Claude W. / Characterization of the basal angiosperm Aristolochia fimbriata : A potential experimental system for genetic studies. In: BMC plant biology. 2013 ; Vol. 13, No. 1.
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title = "Characterization of the basal angiosperm Aristolochia fimbriata: A potential experimental system for genetic studies",
abstract = "Background: Previous studies in basal angiosperms have provided insight into the diversity within the angiosperm lineage and helped to polarize analyses of flowering plant evolution. However, there is still not an experimental system for genetic studies among basal angiosperms to facilitate comparative studies and functional investigation. It would be desirable to identify a basal angiosperm experimental system that possesses many of the features found in existing plant model systems (e.g., Arabidopsis and Oryza).Results: We have considered all basal angiosperm families for general characteristics important for experimental systems, including availability to the scientific community, growth habit, and membership in a large basal angiosperm group that displays a wide spectrum of phenotypic diversity. Most basal angiosperms are woody or aquatic, thus are not well-suited for large scale cultivation, and were excluded. We further investigated members of Aristolochiaceae for ease of culture, life cycle, genome size, and chromosome number. We demonstrated self-compatibility for Aristolochia elegans and A. fimbriata, and transformation with a GFP reporter construct for Saruma henryi and A. fimbriata. Furthermore, A. fimbriata was easily cultivated with a life cycle of just three months, could be regenerated in a tissue culture system, and had one of the smallest genomes among basal angiosperms. An extensive multi-tissue EST dataset was produced for A. fimbriata that includes over 3.8 million 454 sequence reads.Conclusions: Aristolochia fimbriata has numerous features that facilitate genetic studies and is suggested as a potential model system for use with a wide variety of technologies. Emerging genetic and genomic tools for A. fimbriata and closely related species can aid the investigation of floral biology, developmental genetics, biochemical pathways important in plant-insect interactions as well as human health, and various other features present in early angiosperms.",
author = "Bliss, {Barbara J.} and Stefan Wanke and Abdelali Barakat and Saravanaraj Ayyampalayam and Norman Wickett and Wall, {P. Kerr} and Yuannian Jiao and Lena Landherr and Ralph, {Paula E.} and Yi Hu and Christoph Neinhuis and Jim Leebens-Mack and Kathiravetpilla Arumuganathan and Clifton, {Sandra W.} and Maximova, {Siela N.} and Hong Ma and dePamphilis, {Claude W.}",
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Bliss, BJ, Wanke, S, Barakat, A, Ayyampalayam, S, Wickett, N, Wall, PK, Jiao, Y, Landherr, L, Ralph, PE, Hu, Y, Neinhuis, C, Leebens-Mack, J, Arumuganathan, K, Clifton, SW, Maximova, SN, Ma, H & dePamphilis, CW 2013, 'Characterization of the basal angiosperm Aristolochia fimbriata: A potential experimental system for genetic studies', BMC plant biology, vol. 13, no. 1, 13. https://doi.org/10.1186/1471-2229-13-13

Characterization of the basal angiosperm Aristolochia fimbriata : A potential experimental system for genetic studies. / Bliss, Barbara J.; Wanke, Stefan; Barakat, Abdelali; Ayyampalayam, Saravanaraj; Wickett, Norman; Wall, P. Kerr; Jiao, Yuannian; Landherr, Lena; Ralph, Paula E.; Hu, Yi; Neinhuis, Christoph; Leebens-Mack, Jim; Arumuganathan, Kathiravetpilla; Clifton, Sandra W.; Maximova, Siela N.; Ma, Hong; dePamphilis, Claude W.

In: BMC plant biology, Vol. 13, No. 1, 13, 24.01.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of the basal angiosperm Aristolochia fimbriata

T2 - A potential experimental system for genetic studies

AU - Bliss, Barbara J.

AU - Wanke, Stefan

AU - Barakat, Abdelali

AU - Ayyampalayam, Saravanaraj

AU - Wickett, Norman

AU - Wall, P. Kerr

AU - Jiao, Yuannian

AU - Landherr, Lena

AU - Ralph, Paula E.

AU - Hu, Yi

AU - Neinhuis, Christoph

AU - Leebens-Mack, Jim

AU - Arumuganathan, Kathiravetpilla

AU - Clifton, Sandra W.

AU - Maximova, Siela N.

AU - Ma, Hong

AU - dePamphilis, Claude W.

PY - 2013/1/24

Y1 - 2013/1/24

N2 - Background: Previous studies in basal angiosperms have provided insight into the diversity within the angiosperm lineage and helped to polarize analyses of flowering plant evolution. However, there is still not an experimental system for genetic studies among basal angiosperms to facilitate comparative studies and functional investigation. It would be desirable to identify a basal angiosperm experimental system that possesses many of the features found in existing plant model systems (e.g., Arabidopsis and Oryza).Results: We have considered all basal angiosperm families for general characteristics important for experimental systems, including availability to the scientific community, growth habit, and membership in a large basal angiosperm group that displays a wide spectrum of phenotypic diversity. Most basal angiosperms are woody or aquatic, thus are not well-suited for large scale cultivation, and were excluded. We further investigated members of Aristolochiaceae for ease of culture, life cycle, genome size, and chromosome number. We demonstrated self-compatibility for Aristolochia elegans and A. fimbriata, and transformation with a GFP reporter construct for Saruma henryi and A. fimbriata. Furthermore, A. fimbriata was easily cultivated with a life cycle of just three months, could be regenerated in a tissue culture system, and had one of the smallest genomes among basal angiosperms. An extensive multi-tissue EST dataset was produced for A. fimbriata that includes over 3.8 million 454 sequence reads.Conclusions: Aristolochia fimbriata has numerous features that facilitate genetic studies and is suggested as a potential model system for use with a wide variety of technologies. Emerging genetic and genomic tools for A. fimbriata and closely related species can aid the investigation of floral biology, developmental genetics, biochemical pathways important in plant-insect interactions as well as human health, and various other features present in early angiosperms.

AB - Background: Previous studies in basal angiosperms have provided insight into the diversity within the angiosperm lineage and helped to polarize analyses of flowering plant evolution. However, there is still not an experimental system for genetic studies among basal angiosperms to facilitate comparative studies and functional investigation. It would be desirable to identify a basal angiosperm experimental system that possesses many of the features found in existing plant model systems (e.g., Arabidopsis and Oryza).Results: We have considered all basal angiosperm families for general characteristics important for experimental systems, including availability to the scientific community, growth habit, and membership in a large basal angiosperm group that displays a wide spectrum of phenotypic diversity. Most basal angiosperms are woody or aquatic, thus are not well-suited for large scale cultivation, and were excluded. We further investigated members of Aristolochiaceae for ease of culture, life cycle, genome size, and chromosome number. We demonstrated self-compatibility for Aristolochia elegans and A. fimbriata, and transformation with a GFP reporter construct for Saruma henryi and A. fimbriata. Furthermore, A. fimbriata was easily cultivated with a life cycle of just three months, could be regenerated in a tissue culture system, and had one of the smallest genomes among basal angiosperms. An extensive multi-tissue EST dataset was produced for A. fimbriata that includes over 3.8 million 454 sequence reads.Conclusions: Aristolochia fimbriata has numerous features that facilitate genetic studies and is suggested as a potential model system for use with a wide variety of technologies. Emerging genetic and genomic tools for A. fimbriata and closely related species can aid the investigation of floral biology, developmental genetics, biochemical pathways important in plant-insect interactions as well as human health, and various other features present in early angiosperms.

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U2 - 10.1186/1471-2229-13-13

DO - 10.1186/1471-2229-13-13

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